Owens C W, Albuquerque Z P, Tomlinson G M
Gut. 1979 Jul;20(7):568-74. doi: 10.1136/gut.20.7.568.
An original method which uses in vitro anaerobic incubation at 37 degrees C followed by centrifugation, ultrafiltration, and ion exchange chromatography is described; it shows that faecal material suspended in physiological saline can destroy added creatinine. The rate of breakdown by suspensions from uraemic subjects (mean 780 mumol h-1kg-1 SEM 70) was slightly faster than in normal subjects (mean 550 mumol h-1kg-1 SEM 80). Methylamine concentration increased over eight hours as creatinine was metabolised and sarcosine appeared as an intermediate. The rates of these reactions varied within and between individuals and were inhibited by oxygen and centrifugation but not by oxytetracycline. Concentrations of free amino acids did not change significantly despite the formation of ammonia. This approach should be useful in studying the metabolic inter-relationships between intestinal contents and the host organism in health and disease.
描述了一种原始方法，该方法在37℃下进行体外厌氧培养，随后进行离心、超滤和离子交换色谱法；结果表明，悬浮在生理盐水中的粪便物质会破坏添加的肌酐。尿毒症患者悬浮液的分解速率（平均780μmol h-1kg-1，标准误70）略快于正常受试者（平均550μmol h-1kg-1，标准误80）。随着肌酐代谢，甲胺浓度在8小时内升高，肌氨酸作为中间产物出现。这些反应的速率在个体内部和个体之间有所不同，并且受到氧气和离心的抑制，但不受土霉素的抑制。尽管形成了氨，但游离氨基酸的浓度没有显著变化。这种方法在研究健康和疾病状态下肠道内容物与宿主生物体之间的代谢相互关系方面应该是有用的。
