小牛皮肤III型胶原蛋白的共价结构。III. 溴化氰肽α1(III)CB4（第403 - 551位）的氨基酸序列。

The covalent structure of calf skin type III collagen. III. The amino acid sequence of the cyanogen bromide peptide alpha 1(III)CB4 (positions 403--551).

作者信息

Bentz H, Fietzek P P, Kühn K

出版信息

Hoppe Seylers Z Physiol Chem. 1979 Jul;360(7):833-40.

Abstract

The cyanogen bromide peptide alpha 1(III)CB4 comprises the sequence region from position 403 to 551 of the alpha 1(III) chain. Almost the entire sequence of this region was elucidated using two hydroxylamine- and one chymotrypsin-derived fragments for automated Edman degradation. The sequence analysis of alpha 1(III)CB4 was completed with the help of trypsin and one protease V8-derived peptide. Comparison with the corresponding region of the alpha 1(I) chain revealed a striking homology between the two chains in this region which is higher than for the entire alpha chains.

摘要

溴化氰肽α1(III)CB4包含α1(III)链第403位至551位的序列区域。该区域几乎全部序列是利用两个羟胺衍生片段和一个胰凝乳蛋白酶衍生片段进行自动Edman降解确定的。α1(III)CB4的序列分析借助胰蛋白酶和一个蛋白酶V8衍生肽得以完成。与α1(I)链的相应区域比较显示，两条链在该区域具有显著的同源性，高于整个α链的同源性。