肉瘤病毒转化细胞在无血清培养基中的生长及G1期阻滞
Growth and G1 arrest of sarcoma virus trnasformed cells in serum free media.
作者信息
Moskowitz M, Cheng D K
出版信息
J Cell Physiol. 1979 Sep;100(3):589-602. doi: 10.1002/jcp.1041000320.
PMID:489675
Abstract
Rous-sarcoma transformed BHK cells can be continuously cultured in a medium containing Eagle's Minimal Essential Medium, iron and biotin. The rate of cell multiplication increased when serine, or serine plus other non-essential amino-acids were added to the medium. With biotin deleted from the medium there is a reduction in DNA synthesis and most cells are blocked in G1.
摘要
劳斯肉瘤转化的BHK细胞可以在含有伊格尔氏最低限度基本培养基、铁和生物素的培养基中连续培养。当向培养基中添加丝氨酸，或丝氨酸加其他非必需氨基酸时，细胞增殖速率会增加。从培养基中去除生物素后，DNA合成减少，大多数细胞停滞在G1期。
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