Majumdar S K, Udelsman R
J Hered. 1979 May-Jun;70(3):194-8. doi: 10.1093/oxfordjournals.jhered.a109232.
The male antifertility compound, 5-thio-D-glucose, was administered to six groups of C3H/HeJ mice. Three groups received daily intraperitoneal injections of 40 mg/kg for 21, 28, and 35 days, respectively. The other three groups all received injections for 35 days, but were allowed to recover for 21, 42, and 75 days, respectively. The seminiferous tubules of the experimental and control mice were examined and compared with the electron microscope. Histological changes were noted as early as 21 days, with a complete cessation of sperm production by 35 days. The alterations included a reduction in the layers of the germinal epithelium, the formation of giant multinucleated cells, degeneration of germ cells, and the presence of cellular debris in the lumina of the tubules. Accumulation of lysosomes and a dramatic increase of small and large lipid droplets and vacuolar structures morphologically resembling residual bodies containing membranous elements and cellular debris were found in the Sertoli cell cytoplasm. A reinitiation of spermatogenesis was observed in the 21- and 42-days recovery groups. The testicular cells of the 75-day recovery group appeared essentially identical to those of the control animals and had an abundance of sperm.
将雄性抗生育化合物5-硫代-D-葡萄糖给予六组C3H/HeJ小鼠。三组分别每天腹腔注射40mg/kg，持续21天、28天和35天。另外三组均注射35天，但分别允许恢复21天、42天和75天。用电子显微镜检查并比较实验小鼠和对照小鼠的生精小管。早在21天时就注意到组织学变化，到35天时精子产生完全停止。这些改变包括生殖上皮层数减少、巨型多核细胞形成、生殖细胞变性以及小管管腔内存在细胞碎片。在支持细胞胞质中发现溶酶体积累以及大小脂滴和形态上类似于含有膜性成分和细胞碎片的残余体的空泡结构显著增加。在21天和42天恢复组中观察到精子发生重新启动。75天恢复组的睾丸细胞与对照动物的睾丸细胞基本相同，并且有大量精子。
