Hazel J R, Sellner P A
J Exp Zool. 1979 Jul;209(1):105-14. doi: 10.1002/jez.1402090113.
Incorporation of tritium from tritiated water into lipid fractions was measured in isolated hepatocytes from rainbow trout (Salmo gairdneri) acclimated to 5 degrees C and 20 degrees C. Hepatocytes from cold-acclimated trout exhibited significantly higher rates of tritium incorporation into both fatty acid and sterol fractions at assay temperatures of 15 degrees C and 20 degrees C than did hepatocytes from warm-acclimated trout. Tritium incorporation into the fatty acid fraction was nearly temperature independent in hepatocytes from warm-acclimated trout (Q10 = 1.39) but markedly temperature dependent (Q10 = 2.63) in hepatocytes from cold-acclimated trout; in contrast, rates of sterol synthesis were more temperature dependent in warm-acclimated trout. At 5 degrees C, fatty acid lipogenesis comprised a significantly greater percentage of the total tritium incorporation in hepatocytes from warm-acclimated trout and the percentage of total lipogenesis attributable to fatty acids decreased significantly in warm-acclimated trout as the assay temperature increased; the opposite trends were observed in cold-acclimated trout.
在适应5摄氏度和20摄氏度的虹鳟鱼（Salmo gairdneri）分离肝细胞中，测定了氚从氚化水中掺入脂质组分的情况。在15摄氏度和20摄氏度的测定温度下，冷适应鳟鱼的肝细胞中，氚掺入脂肪酸和甾醇组分的速率显著高于热适应鳟鱼的肝细胞。在热适应鳟鱼的肝细胞中，氚掺入脂肪酸组分的情况几乎与温度无关（Q10 = 1.39），但在冷适应鳟鱼的肝细胞中则明显依赖于温度（Q10 = 2.63）；相反，热适应鳟鱼中甾醇合成速率对温度的依赖性更强。在5摄氏度时，脂肪酸脂肪生成在热适应鳟鱼肝细胞中占总氚掺入量的比例显著更高，并且随着测定温度升高，热适应鳟鱼中脂肪酸占总脂肪生成的比例显著下降；在冷适应鳟鱼中观察到相反的趋势。
