Wild T F, Giraudon P, Bernard A, Huppert J
J Med Virol. 1979;4(2):103-14. doi: 10.1002/jmv.1890040205.
A cytopathic measles virus was isolated from a brain biopsy of a subacute sclerosing panencephalitis (SSPE) patient. The agent could be transferred to Vero cells by cocultivation, but the infectivity always remained cell-associated -ie, a defective virus infection. The cell-associated nature of the virus was retained through 25 passages in Vero cells. Intracerebral inoculation of hamsters (2-6 days old) with the cocultured Vero cells gave rise to 100% mortality in 5-7 days. The virus retained its cell-associated nature after passage in hamsters. Electron microscopy of the brain and Vero cocultures showed the presence of virus-like ribonucleoparticles mainly in the nucleus. The presence of viral antigens in the nucleus, cytoplasm, and on the plasma membranes was confirmed by immunofluorescence. Using a combination of immunological and biochemical techniques, it was shown that all the viral proteins were synthesized with the exception of the haemagglutinin. Inclusion of the fusion inhibitor SV4814 (CBZ-D phenylalanine-L-phenylalanine-L-arginine-NO2) in the culture medium led to the elimination of the SSPE infection.
从一名亚急性硬化性全脑炎（SSPE）患者的脑活检组织中分离出一种细胞病变性麻疹病毒。该病原体可通过共培养转移至非洲绿猴肾细胞（Vero细胞），但其感染性始终与细胞相关——即存在缺陷病毒感染。该病毒与细胞的相关性在Vero细胞中传代25次后仍得以保留。用共培养的Vero细胞对仓鼠（2 - 6日龄）进行脑内接种，5 - 7天内死亡率达100%。该病毒在仓鼠体内传代后仍保持其与细胞相关的特性。对脑和Vero细胞共培养物进行电子显微镜检查显示，病毒样核糖核蛋白颗粒主要存在于细胞核中。免疫荧光法证实细胞核、细胞质和质膜上均存在病毒抗原。采用免疫和生化技术相结合的方法表明，除血凝素外，所有病毒蛋白均能合成。在培养基中加入融合抑制剂SV4814（CBZ - D - 苯丙氨酸 - L - 苯丙氨酸 - L - 精氨酸 - NO2）可消除SSPE感染。
