通过酶促错配缬氨酸转移核糖核酸进行密码子识别。
Codon recognition by enzymatically mischarged valine transfer ribonucleic acid.
作者信息
Grunberger D, Weinstein I B, Jacobson K B
出版信息
Science. 1969 Dec 26;166(3913):1635-7. doi: 10.1126/science.166.3913.1635.
Direct evidence for the adaptor hypothesis has been obtained by examining the codon recognition of a purified Escherichia coli valine transfer ribonucleic acid which was enzymatically mischarged with phenylalanine labeled with carbn-14 by reaction with purified phenylalanyl-transfer ribonucleic acid synthetase from Neurospora crassa. The mischarged transfer ribonucleic acid recognized the valine codons but failed to recognize the phenylalanine codon when tested in trinucleotide-directed ribosomal binding assay.
通过检测一种纯化的大肠杆菌缬氨酸转移核糖核酸的密码子识别情况，获得了关于衔接子假说的直接证据。该转移核糖核酸经酶促反应与来自粗糙脉孢菌的纯化苯丙氨酰转移核糖核酸合成酶作用，被错误地带上了用碳 - 14标记的苯丙氨酸。在三核苷酸指导的核糖体结合试验中进行测试时，这种错误负载的转移核糖核酸识别缬氨酸密码子，但不能识别苯丙氨酸密码子。
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