Greenman D L, Mehta R G, Wittliff J L
J Toxicol Environ Health. 1979 Jul;5(4):593-8. doi: 10.1080/15287397909529771.
By using cell-free preparations of uteri obtained from immature BALB/c mice, it was demonstrated that zearalenone and zearalanol, Fusarium mycotoxins, inhibited [3H]estradiol-17 beta binding to specific sites in cytosol. Significant inhibition was noted from zearalenone at 4 x 10(-6) M and from zearalanol at 4 x 10(-7) M. Unlabeled mycotoxins (5 x 10(-6) M) incubated with intact uteri caused translocation of specific estrogen binding sites into nuclei that were exchangeable with [3H]estradiol-17 beta. Zearalanol was more effective in this regard than zearalenone. Ability of the mycotoxins to compete with estradiol-17 beta for the cytosol receptor and to cause translocation of the receptor to the nucleus in general is correlated with their biological activity. These data suggest that the uterotrophic effects of Fusarium mycotoxins are mediated through their association with estrogen receptors in the uterus.
通过使用从未成熟BALB/c小鼠获得的子宫无细胞制剂，已证明镰刀菌属霉菌毒素玉米赤霉烯酮和玉米赤霉醇可抑制[3H]雌二醇-17β与细胞质中特定位点的结合。在4×10^(-6)M的玉米赤霉烯酮和4×10^(-7)M的玉米赤霉醇时观察到显著抑制。与完整子宫一起孵育的未标记霉菌毒素（5×10^(-6)M）导致特定雌激素结合位点易位至可与[3H]雌二醇-17β交换的细胞核中。在这方面，玉米赤霉醇比玉米赤霉烯酮更有效。霉菌毒素与雌二醇-17β竞争细胞质受体并导致受体易位至细胞核的能力通常与其生物活性相关。这些数据表明镰刀菌属霉菌毒素的子宫营养作用是通过它们与子宫中雌激素受体的结合来介导的。
