Tsuji K, Robertson J H, Baas R, McInnis D J
Appl Microbiol. 1969 Sep;18(3):396-8. doi: 10.1128/am.18.3.396-398.1969.
The relative responses of neomycins B and C have been determined by a microbiological agar-diffusion method, a turbidimetric method, and by a recently developed gas-liquid-chromatographic (GLC) method capable of separating the neomycin isomers. The ratios of response of neomycin C to neomycin B by the individual methods were as follows: agar-diffusion method, 1:3; turbidimetric method, 1:2.5; and GLC method, 1:1. When neomycin C is assumed to have 35% biological activity of neomycin B, the calculated drug contents of neomycin sulfate powders obtained by the GLC method correlated well with values obtained by the microbiological agar-diffusion assay method.
通过微生物琼脂扩散法、比浊法以及最近开发的能够分离新霉素异构体的气液色谱（GLC）法，测定了新霉素B和C的相对反应。通过各方法得到的新霉素C与新霉素B的反应比分别如下：琼脂扩散法为1:3；比浊法为1:2.5；GLC法为1:1。当假定新霉素C具有新霉素B 35%的生物活性时，通过GLC法得到的硫酸新霉素粉末的计算药物含量与通过微生物琼脂扩散测定法得到的值相关性良好。
