Breton-Gorius J, Guichard J
Am J Pathol. 1975 Jun;79(3):523-36.
An alkaline diaminobenzidine (DAB) medium has been used to identify peroxidase activity in small granules (0.09 to 0.2 mu in diameter) present in all forms of maturing erythrocytic cells with the exception of erythrocytes. These granules, which were more frequent in proerythroblasts (from two to seven by thin section), were distinct from pleomorphic granules present in the close proximity to the Golgi apparatus. They were also distinct from ferritin molecules which were seen as aggregates in siderosomes of polychromatophilic erythroblasts. They often appeared in close association with the smooth membrane of the nuclear envelope. Optimal conditions for the visualization of these granules by incubation in alkaline DAB were obtained when the peroxidase activity of hemoglobin was reduced by addition of low concentrations of potassium cyanide. Lack of hydrogen peroxide in the incubation media completely inhibited the staining reaction of hemoglobin, while the positive reaction persisted in the granules. Aminotriazole in the incubation media prevented the staining of these organelles. These findings suggest that small granules seen in maturing erythroblasts contain catalase and that they correspond to microperoxisomes described in other tissues. The mechanism of their disappearance during reticulocyte maturation is unknown. The relationship between particulate catalase of erythroblasts and soluble erythrocytic catalase has not been elucidated.
一种碱性二氨基联苯胺（DAB）培养基已被用于鉴定除红细胞外所有成熟红细胞形式中存在的小颗粒（直径0.09至0.2微米）中的过氧化物酶活性。这些颗粒在前成红细胞中更为常见（薄切片观察为2至7个），与高尔基体附近存在的多形性颗粒不同。它们也与在嗜多色性成红细胞的铁小体中聚集成团的铁蛋白分子不同。它们常常与核膜的光滑膜紧密相连。当通过添加低浓度氰化钾降低血红蛋白的过氧化物酶活性时，通过在碱性DAB中孵育来观察这些颗粒的最佳条件得以实现。孵育培养基中缺乏过氧化氢会完全抑制血红蛋白的染色反应，而颗粒中的阳性反应仍然存在。孵育培养基中的氨基三唑可阻止这些细胞器的染色。这些发现表明，在成熟成红细胞中看到的小颗粒含有过氧化氢酶，并且它们与其他组织中描述的微过氧化物酶体相对应。它们在网织红细胞成熟过程中消失的机制尚不清楚。成红细胞的颗粒状过氧化氢酶与可溶性红细胞过氧化氢酶之间的关系尚未阐明。
