苏云金芽孢杆菌核糖体在无细胞苯丙氨酸掺入中对亚精胺的需求。
Spermidine requirement for Bacillus thuringiensis ribosomes in cell-free phenylalanine incorporation.
作者信息
Changchien L M, Aronson J N
出版信息
J Bacteriol. 1970 Sep;103(3):734-40. doi: 10.1128/jb.103.3.734-740.1970.
Abstract
A cell-free system from Bacillus thuringiensis was found to actively incorporate phenylalanine into hot trichloroacetic acid-precipitable material in the presence of synthetic polynucleotide, ribosomes, S-100 supernatant, an energy-generating system, and guanosine triphosphate. Phenylalanine incorporation was absolutely dependent on the presence of spermidine in addition to magnesium ions, even when highly purified ribosomes were used. The spermidine effect could not be attributed to inhibition of nucleases. The ribosomal and supernatant fractions from Escherichia coli and B. thuringiensis could be substituted for each other in this system. The spermidine requirement was shown to be limited to the B. thuringiensis ribosome fraction.
摘要
发现在存在合成多核苷酸、核糖体、S - 100上清液、能量产生系统和三磷酸鸟苷的情况下，来自苏云金芽孢杆菌的无细胞系统能将苯丙氨酸积极地掺入热三氯乙酸可沉淀物质中。即使使用高度纯化的核糖体，苯丙氨酸掺入也绝对依赖于除镁离子外还需亚精胺的存在。亚精胺的作用不能归因于对核酸酶的抑制。在该系统中，大肠杆菌和苏云金芽孢杆菌的核糖体及上清液部分可以相互替代。结果表明，对亚精胺的需求仅限于苏云金芽孢杆菌核糖体部分。
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