荧光素钠和接种培养基对从气溶胶中回收经辐照的大肠杆菌和粘质沙雷氏菌的影响。
Effect of sodium fluorescein and plating medium on recovery of irradiated Escherichia coli and Serratia marcescens from aerosols.
作者信息
Dorsey E L, Berendt R F, Neff E L
出版信息
Appl Microbiol. 1970 Nov;20(5):834-8. doi: 10.1128/am.20.5.834-838.1970.
Abstract
Irradiation of aerosols of either Escherichia coli or Serratia marcescens with simulated solar (xenon) radiation caused a significant decrease in viability. When sodium fluorescein was employed to determine the physical loss of organisms from the aerosol, an additional adverse effect upon survival was noted. The decay curves indicated that at least two mechanisms of inactivation were operative, one due to aerosolization, the other to irradiation. After collection from aerosols, both species of microorganisms grew better on blood agar base than on Casitone agar, but this finding did not appear to be related to the effect of irradiation.
摘要
用模拟太阳（氙气）辐射照射大肠杆菌或粘质沙雷氏菌的气溶胶会导致其活力显著下降。当使用荧光素钠来测定气溶胶中生物体的物理损失时，发现对其存活还有额外的不利影响。衰变曲线表明至少有两种失活机制在起作用，一种是由于气溶胶化，另一种是由于辐射。从气溶胶中收集后，这两种微生物在血琼脂培养基上比在酪蛋白胨琼脂上生长得更好，但这一发现似乎与辐射的影响无关。
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