碱性脑脊髓炎原与组蛋白F2A1的免疫学比较。

Immunological comparison of basic encephalitogen and histone F2A1.

作者信息

Bustin M, Teitelbaum D, Webb C

出版信息

Eur J Biochem. 1975 May 6;53(2):615-21. doi: 10.1111/j.1432-1033.1975.tb04105.x.

DOI:10.1111/j.1432-1033.1975.tb04105.x

Abstract

The extent of immunological cross-reaction between basic encephalitogen and histone F2A1 on both the humoral antibody level and on the cellular level has been established. The extent of humoral cross-reaction was tested by direct complement fixation employing both anti-histone F2A1 and antisera to basic encephalitogen, by inhibition of complement fixation, by radioimmunoassay and by passive cutaneous anaphylaxis. The data obtained failed to reveal immunological cross-reaction at the cellular level was tested by the lymphocyte stimulation technique in rabbits and guinea pigs, by inhibition of lymphocyte stimulation and by delayed hypersensitivity skin reactions. A slight but significant cross-reaction between the two proteins on the cellular level was detected by inhibition of lymphocyte stimulation and by the delayed hypersensitivity test. It is concluded that the immunological studies provide limited evidence that the two proteins share antigenic determinants.

摘要

已确定碱性脑脊髓炎原与组蛋白F2A1在体液抗体水平和细胞水平上的免疫交叉反应程度。通过使用抗组蛋白F2A1和碱性脑脊髓炎原抗血清的直接补体结合试验、补体结合抑制试验、放射免疫测定和被动皮肤过敏反应来检测体液交叉反应程度。所获得的数据未能揭示细胞水平上的免疫交叉反应，通过兔和豚鼠的淋巴细胞刺激技术、淋巴细胞刺激抑制试验和迟发型超敏皮肤反应来检测细胞水平上的免疫交叉反应。通过淋巴细胞刺激抑制试验和迟发型超敏试验检测到这两种蛋白质在细胞水平上存在轻微但显著的交叉反应。得出的结论是，免疫学研究提供了有限的证据表明这两种蛋白质共享抗原决定簇。