Barber T L, Fabricant J
Appl Microbiol. 1971 Apr;21(4):600-5. doi: 10.1128/am.21.4.600-605.1971.
A large collection of avian Mycoplasma cultures was used in studies to improve and develop biological and biochemical characterization techniques. Differential patterns among 11 avian serotypes were shown by carbohydrate fermentation, tetrazolium- and methylene blue-reduction reactions, breakdown of arginine, and the formation of film on egg yolk-agar. Some cultures fermented as many as 14 carbohydrates. Polyhydric alcohols and pentoses were among the compounds fermented. An improved procedure for determining methylene blue reduction by Mycoplasma was developed. These simple, rapid procedures are reproducible and should be useful in grouping Mycoplasma isolates prior to definitive identification by serological or other means.
大量禽支原体培养物被用于研究，以改进和开发生物学及生化特征鉴定技术。通过碳水化合物发酵、四氮唑和亚甲蓝还原反应、精氨酸分解以及在蛋黄琼脂上形成菌膜，显示出11种禽血清型之间的差异模式。一些培养物可发酵多达14种碳水化合物。多元醇和戊糖属于被发酵的化合物。开发了一种改进的支原体亚甲蓝还原测定方法。这些简单、快速的方法具有可重复性，在通过血清学或其他方法进行最终鉴定之前，应用于支原体分离株的分组应该会很有用。
