鸡抗牛血清白蛋白抗体的纯化。
Purification of chicken anti-bovine serum albumin antibody.
作者信息
Schram A C, Hwong S J, Christenson C P
出版信息
Immunology. 1971 Oct;21(4):637-48.
Abstract
Chicken anti-bovine serum albumin antibody was isolated by precipitation with albumin at pH 8.6 in 13 per cent NaCl, solution of the precipitate at pH 11.3–11.5 in 0.9 per cent NaCl, and separation of the antibody from the antigen by gel filtration at the same pH. The purified antibody precipitated its antigen and was precipitated by rabbit antiserum to chicken serum.
摘要
通过在pH 8.6的13%氯化钠溶液中用白蛋白沉淀、在pH 11.3 - 11.5的0.9%氯化钠溶液中溶解沉淀物以及在相同pH下通过凝胶过滤从抗原中分离抗体，分离出鸡抗牛血清白蛋白抗体。纯化后的抗体可沉淀其抗原，并可被兔抗鸡血清沉淀。
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