Crawford I P, Yanofsky C
J Bacteriol. 1971 Oct;108(1):248-53. doi: 10.1128/jb.108.1.248-253.1971.
There is 50% identity in the sequences of the first 50 residues of the alpha chains of Escherichia coli and Pseudomonas putida. No deletions or additions of residues are found in this region, except for the N-terminal methionine residue which is missing in the polypeptide isolated from P. putida. Most of the residues which differ are chemically dissimilar, and half of them are specified by codons which differ by more than a single base. The two residues known by mutational analysis to be essential for catalysis in E. coli are conserved in P. putida. The potential taxonomic usefulness of information of this sort is analyzed.
大肠杆菌和恶臭假单胞菌α链前50个残基的序列有50%的同源性。在该区域未发现残基的缺失或添加,除了从恶臭假单胞菌分离的多肽中缺失的N端甲硫氨酸残基。大多数不同的残基在化学性质上不同,其中一半由相差不止一个碱基的密码子指定。通过突变分析已知对大肠杆菌催化至关重要的两个残基在恶臭假单胞菌中保守。分析了这类信息在分类学上的潜在用途。