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大肠杆菌30S核糖体蛋白之间抗原相似性的分析。

Analysis for antigenic similarities among the 30S ribosomal proteins of Escherichia coli.

作者信息

Strnad B C, Sypherd P S

出版信息

J Bacteriol. 1971 Nov;108(2):910-5. doi: 10.1128/jb.108.2.910-915.1971.

DOI:10.1128/jb.108.2.910-915.1971
PMID:4942770
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC247159/
Abstract

Antiserum was made against a single 30S protein, 30S-7. The amount of complement fixed by total 30S protein in the presence of this antiserum indicated that protein 30S-7 was the major antigen in the mixture of proteins. Each of the 30S ribosomal proteins was tested for cross-reactivity with anti-30S-7. This was done by determining if any of the other 30S proteins inhibited complement fixation by 30S-7. None of the other 30S proteins was found to inhibit complement fixation by 30S-7, indicating that 30S-7 is antigenically distinct from the other proteins.

摘要

制备了针对单一30S蛋白30S - 7的抗血清。在这种抗血清存在的情况下，总30S蛋白所固定的补体量表明，蛋白30S - 7是蛋白质混合物中的主要抗原。对每个30S核糖体蛋白进行了与抗30S - 7交叉反应性的测试。这是通过确定其他任何一种30S蛋白是否抑制30S - 7的补体固定来完成的。未发现其他30S蛋白能抑制30S - 7的补体固定，这表明30S - 7在抗原性上与其他蛋白不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1607/247159/8cd40ab69de6/jbacter00366-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1607/247159/8cd40ab69de6/jbacter00366-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1607/247159/8cd40ab69de6/jbacter00366-0309-a.jpg

相似文献

1
Analysis for antigenic similarities among the 30S ribosomal proteins of Escherichia coli.大肠杆菌30S核糖体蛋白之间抗原相似性的分析。
J Bacteriol. 1971 Nov;108(2):910-5. doi: 10.1128/jb.108.2.910-915.1971.
2
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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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ALTERED ANTIGENICITY OF PEPSINOGEN AND PEPSIN AS AN INDEX OF CONFORMATIONAL CHANGE: EFFECT OF UREA AND REDUCING AGENTS.胃蛋白酶原和胃蛋白酶抗原性改变作为构象变化指标：尿素和还原剂的影响
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ANTIBODIES TO HUMAN A1 HEMOGLOBIN AND THEIR REACTION WITH A2, S, C, AND H HEMOGLOBINS.抗人A1血红蛋白抗体及其与A2、S、C和H血红蛋白的反应
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THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI.大肠杆菌中指定核糖体蛋白的染色体位点。
Proc Natl Acad Sci U S A. 1964 Dec;52(6):1367-74. doi: 10.1073/pnas.52.6.1367.
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Ribosomal proteins of E. Coli: stoichiometry and implications for ribosome structure.大肠杆菌的核糖体蛋白：化学计量及其对核糖体结构的影响。
Cold Spring Harb Symp Quant Biol. 1969;34:25-38. doi: 10.1101/sqb.1969.034.01.007.
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Quantitative immunochemistry and the evolution of primate albumins: micro-complement fixation.定量免疫化学与灵长类动物白蛋白的进化：微量补体结合法
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Properties and interactions of the isolated alpha and beta chains of human haemoglobin. II. Immunochemical behaviour of the chains: fractionation of an antigen.人血红蛋白分离的α链和β链的性质与相互作用。II. 链的免疫化学行为：一种抗原的分级分离
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A ribosomal ambiguity mutation.核糖体模糊突变。
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Cotransduction of strA and ribosomal protein cistrons in Escherichia coli-Salmonella typhimurium hybrids.strA与核糖体蛋白顺反子在大肠杆菌-鼠伤寒沙门氏菌杂种中的共转导
J Bacteriol. 1971 Mar;105(3):947-56. doi: 10.1128/jb.105.3.947-956.1971.
10
Geneti studies of the ribosomal proteins in Escherichi coli. VI. Determination of chromosomal loci for several ribosomal protein components using a hyrid strain between Escherchia coli and Salmonell typhimurium.大肠杆菌核糖体蛋白的遗传学研究。VI. 利用大肠杆菌与鼠伤寒沙门氏菌之间的杂交菌株确定几种核糖体蛋白成分的染色体位点。
Mol Gen Genet. 1970;109(2):131-41. doi: 10.1007/BF00269649.