一种用于膜的密度标记。
A density label for membranes.
作者信息
Fox C F, Law J H, Tsukagoshi N, Wilson G
出版信息
Proc Natl Acad Sci U S A. 1970 Oct;67(2):598-605. doi: 10.1073/pnas.67.2.598.
Abstract
Bromostearic acid satisfies the essential fatty acid requirement of an unsaturated fatty acid auxotroph of Escherichia coli K12. Over 55% of the fatty acids derived from lipids isolated from membranes of cells grown in bromostearate-supplemented medium contained bromine, as established by gas chromatography and mass spectrometry of the methyl esters. During equilibrium centrifugation in sucrose gradients, the cytoplasmic membranes isolated from bromostearate-grown cells banded at a density approximately 0.06 g/cm(3) greater than membranes isolated from oleate- or elaidate-grown cells, affording a complete separation between membranes which contain brominated fatty acids and those which do not.
摘要
溴代硬脂酸满足了大肠杆菌K12不饱和脂肪酸营养缺陷型对必需脂肪酸的需求。通过对甲酯进行气相色谱和质谱分析确定，在添加溴代硬脂酸的培养基中生长的细胞，其细胞膜脂质衍生的脂肪酸中超过55%含有溴。在蔗糖梯度平衡离心过程中，从以溴代硬脂酸为碳源生长的细胞中分离出的细胞质膜，其在蔗糖梯度中的密度比从以油酸或反油酸为碳源生长的细胞中分离出的细胞膜高约0.06 g/cm³，从而实现了含溴脂肪酸的细胞膜与不含溴脂肪酸的细胞膜之间的完全分离。
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本文引用的文献
1
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J Bacteriol. 1950 Jul;60(1):17-28. doi: 10.1128/jb.60.1.17-28.1950.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
A colorimetric method for the determination of sugars.一种测定糖类的比色法。
Nature. 1951 Jul 28;168(4265):167. doi: 10.1038/168167a0.
4
PHOSPHATE BOUND TO HISTIDINE IN A PROTEIN AS AN INTERMEDIATE IN A NOVEL PHOSPHO-TRANSFERASE SYSTEM.蛋白质中与组氨酸结合的磷酸盐作为新型磷转移酶系统中的中间体。
Proc Natl Acad Sci U S A. 1964 Oct;52(4):1067-74. doi: 10.1073/pnas.52.4.1067.
5
METABOLISM AND FUNCTION OF BACTERIAL LIPIDS. I. METABOLISM OF PHOSPHOLIPIDS IN ESCHERICHIA COLI B.细菌脂质的代谢与功能。一、大肠杆菌B中磷脂的代谢
J Biol Chem. 1963 Sep;238:2919-22.
6
Quantitative estimation of peak areas in gas-liquid chromatography.气液色谱中峰面积的定量测定。
Nature. 1961 Jul 22;191:377-8. doi: 10.1038/191377a0.
7
The carbon monoxide compounds of the cytochrome oxidases. I. Difference spectra.细胞色素氧化酶的一氧化碳化合物。I. 差示光谱。
J Biol Chem. 1953 May;202(1):383-96.
8
The effect of fluoride on the succinic oxidase system.氟化物对琥珀酸氧化酶系统的影响。
Biochem J. 1952 Oct;52(2):185-96. doi: 10.1042/bj0520185.
9
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10
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