Fox C F, Law J H, Tsukagoshi N, Wilson G
Proc Natl Acad Sci U S A. 1970 Oct;67(2):598-605. doi: 10.1073/pnas.67.2.598.
Bromostearic acid satisfies the essential fatty acid requirement of an unsaturated fatty acid auxotroph of Escherichia coli K12. Over 55% of the fatty acids derived from lipids isolated from membranes of cells grown in bromostearate-supplemented medium contained bromine, as established by gas chromatography and mass spectrometry of the methyl esters. During equilibrium centrifugation in sucrose gradients, the cytoplasmic membranes isolated from bromostearate-grown cells banded at a density approximately 0.06 g/cm(3) greater than membranes isolated from oleate- or elaidate-grown cells, affording a complete separation between membranes which contain brominated fatty acids and those which do not.
溴代硬脂酸满足了大肠杆菌K12不饱和脂肪酸营养缺陷型对必需脂肪酸的需求。通过对甲酯进行气相色谱和质谱分析确定,在添加溴代硬脂酸的培养基中生长的细胞,其细胞膜脂质衍生的脂肪酸中超过55%含有溴。在蔗糖梯度平衡离心过程中,从以溴代硬脂酸为碳源生长的细胞中分离出的细胞质膜,其在蔗糖梯度中的密度比从以油酸或反油酸为碳源生长的细胞中分离出的细胞膜高约0.06 g/cm³,从而实现了含溴脂肪酸的细胞膜与不含溴脂肪酸的细胞膜之间的完全分离。