补体的杀菌作用与细胞膜流动性之间的关系。
Relationship between bactericidal action of complement and fluidity of cellular membranes.
作者信息
Kato K, Bito Y
出版信息
Infect Immun. 1978 Jan;19(1):12-7. doi: 10.1128/iai.19.1.12-17.1978.
Abstract
The mode of complement-mediated killing of Escherichia coli B cells grown at 25 and 42 degrees C and of E. coli K-12 UFAts cells grown in the presence of oleic and elaidic aicds was examined in relation to their membrane lipid compositions and the thermodynamic properties of membrane phospholipids. Phospholipids isolated from 25 degrees C-grown cells of strain B and from oleic acid-incorporated cells of strain K-12 UFAts had lower phase transition temperatures than did phospholipids from E. coli strain B grown at 42 degrees C or those from strain K-12 UFAts grown on elaidic acid. The rate of cellular susceptibility to complement action closely correlated with the liquid crystalline phase of phospholipids of cell membranes. These findings suggest that membrane fluidity is obligatory for the final expression of complement action.
摘要
研究了在25℃和42℃下生长的大肠杆菌B细胞以及在油酸和反油酸存在下生长的大肠杆菌K - 12 UFAts细胞的补体介导杀伤模式，及其膜脂质组成和膜磷脂的热力学性质之间的关系。从25℃生长的B菌株细胞和掺入油酸的K - 12 UFAts菌株细胞中分离出的磷脂，其相变温度低于在42℃生长的大肠杆菌B菌株或在反油酸上生长的K - 12 UFAts菌株的磷脂。细胞对补体作用的敏感性速率与细胞膜磷脂的液晶相密切相关。这些发现表明，膜流动性是补体作用最终表达所必需的。
相似文献
1
Relationship between bactericidal action of complement and fluidity of cellular membranes.补体的杀菌作用与细胞膜流动性之间的关系。
Infect Immun. 1978 Jan;19(1):12-7. doi: 10.1128/iai.19.1.12-17.1978.
2
Replacement of the aliphatic chains of Clostridium acetobutylicum by exogenous fatty acids: regulation of phospholipid and glycolipid composition.外源脂肪酸对丙酮丁醇梭菌脂肪链的替代：磷脂和糖脂组成的调控
J Bacteriol. 1992 Mar;174(6):1848-53. doi: 10.1128/jb.174.6.1848-1853.1992.
3
Influence of lipids with branched-chain fatty acids on the physical, morphological and functional properties of Escherichia coli cytoplasmic membrane.含支链脂肪酸的脂质对大肠杆菌细胞质膜的物理、形态和功能特性的影响。
Biochim Biophys Acta. 1980 Nov 18;602(3):491-505. doi: 10.1016/0005-2736(80)90328-4.
4
Influence of unsaturated fatty acid membrane component on sensitivity of an Escherichia coli fatty acid auxotroph to conditions of nutrient depletion.不饱和脂肪酸膜成分对大肠杆菌脂肪酸营养缺陷型菌株对营养耗尽条件敏感性的影响。
Appl Environ Microbiol. 1988 Aug;54(8):2107-11. doi: 10.1128/aem.54.8.2107-2111.1988.
5
A density label for membranes.一种用于膜的密度标记。
Proc Natl Acad Sci U S A. 1970 Oct;67(2):598-605. doi: 10.1073/pnas.67.2.598.
6
7
Fatty acid incorporation into membranes of dispersed rat submandibular salivary gland cells and their effect on adenylate cyclase activity.
Arch Oral Biol. 1990;35(11):879-83. doi: 10.1016/0003-9969(90)90066-j.
8
Phase transitions of phospholipid bilayers from an unsaturated fatty acid auxotroph of Escherichia coli.来自大肠杆菌不饱和脂肪酸营养缺陷型的磷脂双层的相变
Biochim Biophys Acta. 1977 May 2;466(3):393-401. doi: 10.1016/0005-2736(77)90332-7.
9
Isolation and properties of complement-resistant strains of Escherichia coli K-12.大肠杆菌K-12补体抗性菌株的分离与特性
Infect Immun. 1977 Nov;18(2):446-53. doi: 10.1128/iai.18.2.446-453.1977.
10
Induction of an endothermic transition in the Arrhenius plot of fatty acid uptake by lipid-depleted ascites tumor cells.
Biochim Biophys Acta. 1989 Sep 11;1005(1):27-33. doi: 10.1016/0005-2760(89)90027-1.
引用本文的文献
1
On the lysis of paroxysmal nocturnal hemoglobinuria erythrocytes by complement: dual role of C3b.补体对阵发性夜间血红蛋白尿红细胞的溶解作用：C3b的双重作用
Blut. 1982 Oct;45(4):249-59. doi: 10.1007/BF00320192.
2
Analysis of outer membrane components of Escherichia coli ML308 225 and of a serum-resistant mutant.大肠杆菌ML308 225及血清抗性突变体的外膜成分分析。
Infect Immun. 1980 May;28(2):387-92. doi: 10.1128/iai.28.2.387-392.1980.
3
Effect of agents that produce membrane disorder on lysis of erythrocytes by complement.产生膜紊乱的试剂对补体介导的红细胞溶解的影响。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2522-5. doi: 10.1073/pnas.78.4.2522.
4
Is the membrane attack complex of complement an enzyme?补体的膜攻击复合物是一种酶吗？
Mol Cell Biochem. 1984;61(1):5-15. doi: 10.1007/BF00239603.
5
Bactericidal and bacteriolytic activity of serum against gram-negative bacteria.血清对革兰氏阴性菌的杀菌和溶菌活性。
Microbiol Rev. 1983 Mar;47(1):46-83. doi: 10.1128/mr.47.1.46-83.1983.
6
Synthesis of lipids or lipid-containing macromolecules in tumor cells. Relevance to host defense.肿瘤细胞中脂质或含脂质大分子的合成。与宿主防御的相关性。
Surv Immunol Res. 1983;2(2):122-8. doi: 10.1007/BF02918569.
7
Inhibition of serum bactericidal reaction by lipopolysaccharide.脂多糖对血清杀菌反应的抑制作用。
Infect Immun. 1985 Jun;48(3):759-62. doi: 10.1128/iai.48.3.759-762.1985.
8
Determination of serum bactericidal activity against Escherichia coli by an automated photometric method.采用自动光度法测定血清对大肠杆菌的杀菌活性。
J Clin Microbiol. 1988 Oct;26(10):2069-76. doi: 10.1128/jcm.26.10.2069-2076.1988.
本文引用的文献
1
KINETICS OF THE BACTERICIDAL ACTION OF NORMAL SERUM ON GRAM-NEGATIVE BACTERIA.正常血清对革兰氏阴性菌杀菌作用的动力学
J Immunol. 1965 Jul;95:75-9.
2
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
3
[Structure of liquid-crystalline phases of different phospholipids, monoglycerides, sphingolipids in the absence or presence of water].[不同磷脂、甘油单酯、鞘脂在无水或有水情况下液晶相的结构]
J Mol Biol. 1967 May 14;25(3):363-82. doi: 10.1016/0022-2836(67)90192-1.
4
Physical studies of phospholipids. II. Monolayer studies of some synthetic 2,3-diacyl-DL-phosphatidylethanolamines and phosphatidylcholines containing trans double bonds.磷脂的物理研究。II. 一些含反式双键的合成2,3-二酰基-DL-磷脂酰乙醇胺和磷脂酰胆碱的单分子层研究。
Biochim Biophys Acta. 1966 May 12;120(1):148-55. doi: 10.1016/0926-6585(66)90286-x.
5
Polymorphism of lecithins.卵磷脂的多态性。
Nature. 1968 Jun 15;218(5146):1031-4. doi: 10.1038/2181031a0.
6
Fate of phospholipids in liposomal model membranes damaged by antibody and complement.抗体和补体损伤的脂质体模型膜中磷脂的命运
Biochemistry. 1970 Nov 24;9(24):4767-76. doi: 10.1021/bi00826a021.
7
Calorimetric evidence for the liquid-crystalline state of lipids in a biomembrane.生物膜中脂质液晶态的量热学证据。
Proc Natl Acad Sci U S A. 1969 May;63(1):104-9. doi: 10.1073/pnas.63.1.104.
8
The molecular organization of lipids in the membrane of Escherichia coli: phase transitions.大肠杆菌膜中脂质的分子组织：相变
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3180-4. doi: 10.1073/pnas.68.12.3180.
9
Lipids containing trans-unsaturated fatty acids change the temperature characteristic of thiomethylgalactoside accumulation in Escherichia coli.含有反式不饱和脂肪酸的脂质会改变大肠杆菌中硫代甲基半乳糖苷积累的温度特性。
J Mol Biol. 1969 Aug 28;44(1):209-14. doi: 10.1016/0022-2836(69)90416-1.
10
Fatty acid mutant of E. coli lacking a beta-hydroxydecanoyl thioester dehydrase.缺乏β-羟基癸酰硫酯脱水酶的大肠杆菌脂肪酸突变体。
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1579-86. doi: 10.1073/pnas.58.4.1579.
Zotero 插件