补体的杀菌作用与细胞膜流动性之间的关系。
Relationship between bactericidal action of complement and fluidity of cellular membranes.
作者信息
Kato K, Bito Y
出版信息
Infect Immun. 1978 Jan;19(1):12-7. doi: 10.1128/iai.19.1.12-17.1978.
Abstract
The mode of complement-mediated killing of Escherichia coli B cells grown at 25 and 42 degrees C and of E. coli K-12 UFAts cells grown in the presence of oleic and elaidic aicds was examined in relation to their membrane lipid compositions and the thermodynamic properties of membrane phospholipids. Phospholipids isolated from 25 degrees C-grown cells of strain B and from oleic acid-incorporated cells of strain K-12 UFAts had lower phase transition temperatures than did phospholipids from E. coli strain B grown at 42 degrees C or those from strain K-12 UFAts grown on elaidic acid. The rate of cellular susceptibility to complement action closely correlated with the liquid crystalline phase of phospholipids of cell membranes. These findings suggest that membrane fluidity is obligatory for the final expression of complement action.
摘要
研究了在25℃和42℃下生长的大肠杆菌B细胞以及在油酸和反油酸存在下生长的大肠杆菌K - 12 UFAts细胞的补体介导杀伤模式，及其膜脂质组成和膜磷脂的热力学性质之间的关系。从25℃生长的B菌株细胞和掺入油酸的K - 12 UFAts菌株细胞中分离出的磷脂，其相变温度低于在42℃生长的大肠杆菌B菌株或在反油酸上生长的K - 12 UFAts菌株的磷脂。细胞对补体作用的敏感性速率与细胞膜磷脂的液晶相密切相关。这些发现表明，膜流动性是补体作用最终表达所必需的。
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