Dayal B, Bagan E, Tint G S, Shefer S, Salen G
Steroids. 1979 Sep;34(3):259-72. doi: 10.1016/0039-128x(79)90078-3.
[3beta-3H]-bile acids and bile alcohols may be useful for metabolic studies in man and animals because the 3-position is invulnerable to bacterial attack. A number of tritium labeled bile acids and bile alcohols were prepared by selective oxidation of the hydroxyl group at carbon-3 followed by reduction with NaBT4. In each case, the bile acids and bile alcohols epimeric at carbon-3 were resolved by analytical and preparative thin-layer chromatography and characterized by gasliquid chromatography. The average yield was 60--65% and specific activities of the final products were in the range of 7.4 x 10(7) dpm/mg.
[3β-³H]胆汁酸和胆汁醇可能对人和动物的代谢研究有用，因为3位对细菌攻击具有抗性。通过对碳-3位的羟基进行选择性氧化，然后用NaBT₄还原，制备了多种氚标记的胆汁酸和胆汁醇。在每种情况下，通过分析型和制备型薄层色谱法拆分了在碳-3位差向异构的胆汁酸和胆汁醇，并通过气相色谱法进行了表征。平均产率为60%-65%，最终产物的比活度在7.4×10⁷ dpm/mg范围内。
