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从蛋白质合成抑制中恢复的细胞中诱导型和组成型β-半乳糖苷酶的形成。

Inducible and constitutive -galactosidase formation in cells recovering from protein synthesis inhibition.

作者信息

Soreq H, Kaplan R

出版信息

J Bacteriol. 1971 Dec;108(3):1147-53. doi: 10.1128/jb.108.3.1147-1153.1971.

Abstract

Inducible and constitutive beta-galactosidase formation and radioactive amino acid incorporation were measured in cells recovering from various treatments which inhibit protein synthesis in the cell. Undelayed beta-galactosidase formation was found in stringent auxotrophs recovering from amino acid starvation, in cells recovering from glycerol or potassium starvation, and in bacteria recovering from puromycin treatment. Delayed beta-galactosidase formation was found in relaxed auxotrophs recovering from amino acid starvation and in prototrophs recovering from chloramphenicol or from tetracycline treatment. The length of this delay was directly proportional to the duration of the treatment. All cells recovering from the various treatments exhibited a slightly decreased rate of beta-galactosidase formation and an increase in radioactive amino acid incorporation.

摘要

在从各种抑制细胞蛋白质合成的处理中恢复的细胞中,测定了诱导型和组成型β-半乳糖苷酶的形成以及放射性氨基酸的掺入。在从氨基酸饥饿中恢复的严格营养缺陷型细胞、从甘油或钾饥饿中恢复的细胞以及从嘌呤霉素处理中恢复的细菌中,发现β-半乳糖苷酶的形成没有延迟。在从氨基酸饥饿中恢复的松弛营养缺陷型细胞以及从氯霉素或四环素处理中恢复的原养型细胞中,发现β-半乳糖苷酶的形成有延迟。这种延迟的长度与处理的持续时间成正比。从各种处理中恢复的所有细胞都表现出β-半乳糖苷酶形成速率略有下降以及放射性氨基酸掺入增加。

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