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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1971 Dec;125(3):721-32. doi: 10.1042/bj1250721.

Rat lymph-node cells were incubated in serum and medium 199 with [5-(3)H]uridine or [5-(3)H]cytidine and acid-precipitable radioactivity was measured. Results were interpreted in terms of an isotope-dilution model. 2. Both serum and medium 199 contained pools that inhibited radioactive labelling in a competitive manner. The serum activity was diffusible and inhibited labelling with [(3)H]cytidine more than with [(3)H]uridine; in these respects the activity resembled cytidine (14mum). 3. The pools in serum and plasma were the same size; however, the rate of labelling was greater in plasma, owing to a diffusible factor. 4. Paradoxically, relatively simple media (Earle's salts and Eagle's minimum essential) appeared to have a larger pool than the more complex pyrimidine-containing medium 199; this suggests a contribution to the pool by cells in the simple media. 5. In the absence of pools the average cell was capable of incorporating 2000 radioactive nucleoside molecules/s.

将大鼠淋巴结细胞置于含有[5-(3)H]尿苷或[5-(3)H]胞苷的血清和199培养基中孵育，然后测定酸沉淀放射性。结果根据同位素稀释模型进行解释。2. 血清和199培养基均含有以竞争方式抑制放射性标记的库。血清活性是可扩散的，对[(3)H]胞苷标记的抑制作用比对[(3)H]尿苷的抑制作用更强；在这些方面，该活性类似于胞苷(14μm)。3. 血清和血浆中的库大小相同；然而，由于一个可扩散因子，血浆中的标记率更高。4. 矛盾的是，相对简单的培养基（Earle盐和Eagle最低必需培养基）似乎比更复杂的含嘧啶的199培养基有更大的库；这表明简单培养基中的细胞对该库有贡献。5. 在没有库的情况下，平均每个细胞能够每秒掺入2000个放射性核苷分子。

Biochem J. 1971 Dec;125(3):721-32. doi: 10.1042/bj1250721.

Rat lymph-node cells were incubated in serum and medium 199 with [5-(3)H]uridine or [5-(3)H]cytidine and acid-precipitable radioactivity was measured. Results were interpreted in terms of an isotope-dilution model. 2. Both serum and medium 199 contained pools that inhibited radioactive labelling in a competitive manner. The serum activity was diffusible and inhibited labelling with [(3)H]cytidine more than with [(3)H]uridine; in these respects the activity resembled cytidine (14mum). 3. The pools in serum and plasma were the same size; however, the rate of labelling was greater in plasma, owing to a diffusible factor. 4. Paradoxically, relatively simple media (Earle's salts and Eagle's minimum essential) appeared to have a larger pool than the more complex pyrimidine-containing medium 199; this suggests a contribution to the pool by cells in the simple media. 5. In the absence of pools the average cell was capable of incorporating 2000 radioactive nucleoside molecules/s.

将大鼠淋巴结细胞置于含有[5-(3)H]尿苷或[5-(3)H]胞苷的血清和199培养基中孵育，然后测定酸沉淀放射性。结果根据同位素稀释模型进行解释。2. 血清和199培养基均含有以竞争方式抑制放射性标记的库。血清活性是可扩散的，对[(3)H]胞苷标记的抑制作用比对[(3)H]尿苷的抑制作用更强；在这些方面，该活性类似于胞苷(14μm)。3. 血清和血浆中的库大小相同；然而，由于一个可扩散因子，血浆中的标记率更高。4. 矛盾的是，相对简单的培养基（Earle盐和Eagle最低必需培养基）似乎比更复杂的含嘧啶的199培养基有更大的库；这表明简单培养基中的细胞对该库有贡献。5. 在没有库的情况下，平均每个细胞能够每秒掺入2000个放射性核苷分子。