Nielsen J
Acta Endocrinol (Copenh). 1979 Aug;91(4):680-91. doi: 10.1530/acta.0.0910680.
Antibodies against cortisol-21-hemisuccinate conjugated to bovine serum albumin was raised in rabbits and conjugated with poly-maleic acid anhydride. The conjugate was used in a solid phase radioimmunoassay to measure the urinary excretion of unconjugated cortisol. Cortisol was extracted with methylene chloride, the extract purified by chromatography on Sephadex LH-20, and the cortisol content measured with the radioimmunoassay. The intra-assay variation of the method was 6 per cent and the interassay variation 10 per cent. In 95 normal adult persons the excretion of free cortisol was 11--70, mean 30 micrograms/24 h. No sex or age difference was found. In 8 children aged 7--14 years the excretion was 8--46 micrograms/24 h.
将与牛血清白蛋白偶联的皮质醇-21-半琥珀酸抗体在兔体内产生，并与聚马来酸酐偶联。该偶联物用于固相放射免疫测定以测量未结合皮质醇的尿排泄量。用二氯甲烷提取皮质醇，提取物通过在Sephadex LH-20上的色谱法纯化，并用放射免疫测定法测量皮质醇含量。该方法的批内变异为6%，批间变异为10%。在95名正常成年人中，游离皮质醇的排泄量为11--70，平均30微克/24小时。未发现性别或年龄差异。在8名7--14岁的儿童中，排泄量为8--46微克/24小时。
