网织红细胞核糖体分离与分级分离方法的比较
A comparison of methods for the isolation and fractionation of reticulocyte ribosomes.
作者信息
Arnstein H R, Cox R A, Gould H, Potter H
出版信息
Biochem J. 1965 Aug;96(2):500-6. doi: 10.1042/bj0960500.
Abstract
- Polysomes, ribosomes and pH5 enzymes were isolated from rabbit reticulocytes by acidifying the post-mitochondrial supernatant to pH6.0 to precipitate all ribonucleoprotein particles and about half the pH5 enzymes; the precipitate was redissolved in buffer, pH7.6, and fractionated by zone centrifuging. 2. The isolation of polysome-rich and ribosome-rich fractions from the post-mitochondrial supernatant was also examined. 3. Studies of the stability of polysomes revealed that dissociation into sub-units occurred when both bound and free Mg(2+) was chelated by EDTA or when the pH was increased above pH8.8.
摘要
- 通过将线粒体后上清液酸化至pH6.0，沉淀所有核糖核蛋白颗粒和约一半的pH5酶，从兔网织红细胞中分离出多核糖体、核糖体和pH5酶；将沉淀重新溶解于pH7.6的缓冲液中，并通过区带离心进行分级分离。2. 还研究了从线粒体后上清液中分离富含多核糖体和富含核糖体的组分。3. 对多核糖体稳定性的研究表明，当结合态和游离态的Mg(2+)都被EDTA螯合或pH升高至pH8.8以上时，多核糖体会解离成亚基。
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