枯草芽孢杆菌噬菌体的特性分析。
Characterization of Bacillus subtilis bacteriophages.
作者信息
Brodetsky A M, Romig W R
出版信息
J Bacteriol. 1965 Dec;90(6):1655-63. doi: 10.1128/jb.90.6.1655-1663.1965.
Brodetsky, Anna M. (University of California, Los Angeles), and W. R. Romig. Characterization of Bacillus subtilis bacteriophages. J. Bacteriol. 90:1655-1663. 1965.-A group of six phages, SP5, SP6, SP7, SP8, SP9, and SP13, which use the Marburg strain of Bacillus subtilis as host was characterized. These phages, referred to as group 1, were examined for the following properties: host range, plaque morphology, stability, adsorption kinetics, one-step growth characteristics, calcium requirements, serum neutralization, thermal inactivation, and inactivation by ultraviolet irradiation. Five unrelated B. subtilis phages, SP3, SP10, PBS1, SP alpha, and SP beta, were included in the studies. When first isolated, none of the group 1 phages was able to replicate efficiently on B. subtilis SB19, a mutant of the "transforming" B. subtilis 168. Host range mutants capable of growth in SB19 were isolated for all of the group 1 phages except SP13, and are designated the "star" phages (SP5* through SP9*). For characterization, SB19 was used as host for the star phages, and another B. subtilis mutant, 168B, was host for SP13.
布罗茨基，安娜·M.（加利福尼亚大学洛杉矶分校）和W.R.罗米格。枯草芽孢杆菌噬菌体的特性。《细菌学杂志》90:1655 - 1663。1965年。——对一组以枯草芽孢杆菌马尔堡菌株为宿主的六种噬菌体SP5、SP6、SP7、SP8、SP9和SP13进行了特性鉴定。这些噬菌体被称为第1组，对其进行了以下特性检测：宿主范围、噬菌斑形态、稳定性、吸附动力学、一步生长特性、钙需求、血清中和、热灭活以及紫外线照射灭活。研究中还包括五种不相关的枯草芽孢杆菌噬菌体SP3、SP10、PBS1、SPα和SPβ。最初分离时，第1组噬菌体中没有一种能够在枯草芽孢杆菌168的“转化”突变体SB19上高效复制。除SP13外，所有第1组噬菌体都分离出了能够在SB19中生长的宿主范围突变体，这些突变体被称为“星”噬菌体（SP5至SP9）。为了进行特性鉴定，SB19被用作“星”噬菌体的宿主，另一种枯草芽孢杆菌突变体168B被用作SP13的宿主。
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