Pinckard R N, Weir D M
Clin Exp Immunol. 1966 Jan;1(1):33-43.
The liver antigen which fixes complement with rat serum containing the IgM antibody induced by carbon tetrachloride (CCl) injection is primarily associated with the `mitochondrial fraction' of liver. The extreme lability of the antigen precluded any attempt to isolate a specific protein, lipoprotein or lipopolysaccharide moiety. This was considered to be due to the presence of lysosomes which would be likely to autolyse the antigenic components. It was concluded that the particulate nature of the antigen explained the finding of antibody activity in the IgM fraction of serum and that the inability to induce tolerance to the antigen also depended on its particulate nature. It is suggested that the individual may not develop tolerance to particulate subcellular components.
与注射四氯化碳（CCl）诱导产生的含IgM抗体的大鼠血清补体结合的肝脏抗原，主要与肝脏的“线粒体部分”相关。该抗原的极度不稳定性使得分离特定蛋白质、脂蛋白或脂多糖部分的任何尝试都无法进行。这被认为是由于存在溶酶体，溶酶体可能会自溶抗原成分。得出的结论是，抗原的颗粒性质解释了在血清IgM部分发现抗体活性的现象，并且无法诱导对抗原的耐受性也取决于其颗粒性质。有人提出，个体可能不会对颗粒状亚细胞成分产生耐受性。
