Kessel R W, Freedman H H, Braun W
J Bacteriol. 1966 Sep;92(3):592-6. doi: 10.1128/jb.92.3.592-596.1966.
Kessel, R. W. I. (Rutgers, The State University, New Brunswick, N.J.), Henry H. Freedman, and Werner Braun. Relation of polysaccharide content to some biological properties of endotoxins from mutants of Salmonella typhimurium. J. Bacteriol. 92:592-596. 1966.-Endotoxins were extracted by the phenol-water procedure from a variety of Salmonella typhimurium mutants with known differences in the composition of their cell wall polysaccharides. The lethality of these preparations for mice proved to be correlated with the complexity of the polysaccharide: endotoxin from the smooth parent strain and from rough strains with several sugars attached to the heptose-phosphate backbone were of high toxicity, whereas endotoxin from a mutant possessing only glucose attached to the heptose-phosphate backbone was less toxic, and endotoxin from a mutant possessing the backbone only was least toxic. All of these mutants yielded endotoxins that were equally capable of protecting mice against subsequent challenge with Pseudomonas aeruginosa. Material obtained from a heptoseless mutant by the phenol-water method proved to be neither toxic nor protective. The apparent dissociation of biological properties that can be achieved with the aid of endotoxin preparations from certain mutants is discussed in terms of possible mechanisms.
凯塞尔，R. W. I.（罗格斯州立大学，新不伦瑞克，新泽西州），亨利·H. 弗里德曼，以及维尔纳·布劳恩。鼠伤寒沙门氏菌突变体内毒素的多糖含量与某些生物学特性的关系。《细菌学杂志》92:592 - 596。1966年。——采用酚水法从多种鼠伤寒沙门氏菌突变体中提取内毒素，这些突变体的细胞壁多糖组成已知存在差异。结果证明，这些制剂对小鼠的致死性与多糖的复杂性相关：来自光滑亲本菌株以及来自庚糖 - 磷酸主链上连接有几种糖的粗糙菌株的内毒素毒性高，而来自仅在庚糖 - 磷酸主链上连接有葡萄糖的突变体的内毒素毒性较小，来自仅具有主链的突变体的内毒素毒性最小。所有这些突变体产生的内毒素在保护小鼠免受随后铜绿假单胞菌攻击方面能力相同。通过酚水法从无庚糖突变体获得的物质既无毒性也无保护作用。借助某些突变体的内毒素制剂可实现的生物学特性的明显分离，从可能的机制方面进行了讨论。
