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流式细胞光度法测定精子DNA分析

[Analysis of spermatozoa with flow cytophotometric determination of DNA].

作者信息

Otto F J, Hofmann N, Hettwer H, Oldiges H

出版信息

Andrologia. 1979 Jul-Aug;11(4):279-86.

PMID:496033
Abstract

Semen samples of fertility patients and fertile men were analysed by an improved technique of flow cytophotometry. The DNA-contents of mature spermatozoa were measured with a high level of accuracy. Besides spermatozoa, peaks of spermiogenetic cells and other seminal cells could be demonstrated. Complementary to the results of semen analyses the complex histograms of pulse cytophotometry investigations may give further informations on the nature of the underlying disturbances of spermatogenesis.

摘要

采用改良的流式细胞光度术对不育患者和生育男性的精液样本进行分析。可高精度测量成熟精子的DNA含量。除精子外,还可显示精子发生细胞和其他精细胞的峰值。作为精液分析结果的补充,脉冲细胞光度术研究的复杂直方图可能会提供有关精子发生潜在紊乱本质的更多信息。

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