Pasqualotto Fabio Firmbach, Sundaram Arjun, Sharma Rakesh Kumar, Borges Edson, Pasqualotto Eleanora Bedin, Agarwal Ashok
Center for Biological and Health Sciences, University of Caxias do Sul, Caxias do Sul, Brazil.
Fertil Steril. 2008 Mar;89(3):602-7. doi: 10.1016/j.fertnstert.2007.03.057. Epub 2007 May 7.
To compare semen quality and levels of seminal oxidative stress among three groups: infertile men with varicocele, fertile men with varicocele, and healthy semen donors (controls) without varicocele.
Prospective study.
Academic medical centers.
INTERVENTION(S): None.
PATIENT(S): Semen specimens were obtained from 21 infertile patients with varicocele, 15 fertile men with varicocele, and 17 healthy fertile men with normal semen characteristics.
MAIN OUTCOME MEASURE(S): Principal component analysis was applied to nine semen characteristics to provide a standardized semen quality score. Reactive oxygen species (ROS) production and total antioxidant capacity (TAC) were measured by chemiluminescence assays to create an ROS-TAC score.
RESULT(S): The mean semen quality scores of the infertile patients with varicocele were lower than those of the control subjects but similar to those of the fertile men with varicocele. Compared with the healthy subjects, the infertile men with varicocele had higher ROS levels but lower TAC levels. They also had significantly lower ROS-TAC scores compared with control subjects, but the scores were not significantly different than those seen in fertile men with varicocele.
CONCLUSION(S): These findings not only provide us with valuable information regarding semen quality but also can serve as a warning that the fertility potential in fertile varicocele patients can decline due to oxidative stress.
比较三组人群的精液质量和精液氧化应激水平，这三组分别为：患有精索静脉曲张的不育男性、患有精索静脉曲张的可育男性以及无精索静脉曲张的健康精液捐献者（对照组）。
前瞻性研究。
学术医学中心。
无。
从21名患有精索静脉曲张的不育患者、15名患有精索静脉曲张的可育男性以及17名精液特征正常的健康可育男性中获取精液样本。
对九项精液特征进行主成分分析，以提供标准化的精液质量评分。通过化学发光法测定活性氧（ROS）生成量和总抗氧化能力（TAC），以得出ROS - TAC评分。
患有精索静脉曲张的不育患者的平均精液质量评分低于对照组，但与患有精索静脉曲张的可育男性相似。与健康受试者相比，患有精索静脉曲张的不育男性的ROS水平较高，但TAC水平较低。与对照组相比，他们的ROS - TAC评分也显著更低，但与患有精索静脉曲张的可育男性的评分无显著差异。
这些发现不仅为我们提供了有关精液质量的有价值信息，还警示我们，患有精索静脉曲张的可育患者的生育潜力可能因氧化应激而下降。
