细菌芽孢形成的生化研究。IV. 巨大芽孢杆菌营养细胞和芽孢的无机焦磷酸酶
Biochemical studies of bacterial sporulation. IV. Inorganic pyrophosphatase of vegetative cells and spores of Bacillus megaterium.
作者信息
Tono H, Kornberg A
出版信息
J Bacteriol. 1967 Jun;93(6):1819-24. doi: 10.1128/jb.93.6.1819-1824.1967.
Abstract
Inorganic pyrophosphatase activities extracted from vegetative cells and spores of Bacillus megaterium were compared and found to be similar in behavior on polyacrylamide gel electrophoresis, in metal and pH requirements for activity, and in response to inhibitors. In the sporulating cell, an additional electrophoretic species of the enzyme was observed which could be partially converted to the principal form by treatment with Mn(++); both forms of the enzyme required Mn(++) for stabilization in solution as well as for activity.
摘要
对从巨大芽孢杆菌的营养细胞和孢子中提取的无机焦磷酸酶活性进行了比较，发现它们在聚丙烯酰胺凝胶电泳中的行为、活性对金属和pH的要求以及对抑制剂的反应方面相似。在芽孢形成细胞中，观察到该酶的另一种电泳形式，用Mn(++)处理后可部分转化为主要形式；两种形式的酶在溶液中稳定以及发挥活性都需要Mn(++)。
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