Novikova T E, Nechaeva N V
Arkh Anat Gistol Embriol. 1979 Sep;77(9):103-7.
"In vitro" incubated slices of the rat liver, by the method of interferometry combined with radioautography, have demonstrated hour's rhythmic fluctuations in protein concentration and 3H-lysine incorporation into proteins. The biochemical method has demonstrated fluctuations in protein synthesis rate in the same cells with an average period of about one hour. The possibility to apply the interferometric method in combination with radioautography for revealing quantitative changes of protein in slices of organic cultures is demonstrated.
通过干涉测量法与放射自显影法相结合的方法,对大鼠肝脏的“体外”孵育切片进行研究,结果表明蛋白质浓度以及3H-赖氨酸掺入蛋白质的过程存在每小时的节律性波动。生化方法已证明同一细胞中蛋白质合成速率存在波动,平均周期约为1小时。本文证明了将干涉测量法与放射自显影法结合应用于揭示有机培养切片中蛋白质定量变化的可能性。