Brown O R, Huggett D O
Appl Microbiol. 1968 Mar;16(3):476-9. doi: 10.1128/am.16.3.476-479.1968.
A membrane culture technique was developed for directly exposing microorganisms to test atmospheres. Inhibition and killing were calculated from comparisons with air-grown cultures. Direct colony counts were used with low inocula. With mass inocula, plate colony counts and optical-density measurements were made on resuspended filter populations. Bacteria, including Escherichia coli, were more sensitive to oxygen than previously reported. With inocula of a few hundred cells per membrane, five of seven species failed to produce colonies while exposed to oxygen at one atmosphere. Upon reincubation in air, the survival of five species ranged from near 0 to 12% of the cells. Aerobacter aerogenes was neither inhibited nor killed. With this technique, bacteria are in direct contact with the test atmosphere and cells which survive are detected but do not obscure the response of other cells in the population.
开发了一种膜培养技术，用于将微生物直接暴露于测试气氛中。通过与在空气中生长的培养物进行比较来计算抑制率和杀灭率。低接种量时使用直接菌落计数法。对于大量接种物，对重悬的滤膜菌群体进行平板菌落计数和光密度测量。包括大肠杆菌在内的细菌对氧气比先前报道的更敏感。当每个膜接种几百个细胞时，七种细菌中有五种在一个大气压下暴露于氧气时未能产生菌落。在空气中再次培养后，五种细菌的存活率在接近0%至12%的细胞范围内。产气气杆菌既未被抑制也未被杀死。使用这种技术，细菌与测试气氛直接接触，存活的细胞可以被检测到，但不会掩盖群体中其他细胞的反应。
