Stottmeier K D, Woodley C L, Kubica G P, Beam R E
Bull World Health Organ. 1967;37(6):961-6.
The authors describe the use of the vertical diffusion test for determining the quantity of certain tuberculostatic drugs in body fluids and the degree of mycobacterial susceptibility to these drugs, using tubercle bacilli as test organisms. It is found to be a reliable assay method for isoniazid, para-aminosalicylic acid, ethionamide and ethambutol when carried out on a modification of Middlebrook 7H-10 solid medium containing 1.5% Oxoid Ion-Agar No. 2.As a drug-susceptibility test, it yields results expressed quantitatively in degrees of sensitivity or resistance and is therefore recommended for use only in laboratories not equipped for more comprehensive testing. The method may also be used for ascertaining the level of tuberculostatic activity of a patient's serum against his own mycobacteria.
作者描述了使用垂直扩散试验来测定体液中某些抗结核药物的含量以及结核分枝杆菌对这些药物的敏感程度，以结核杆菌作为测试微生物。当在含有1.5% Oxoid 2号离子琼脂的改良Middlebrook 7H - 10固体培养基上进行时，发现该试验对于异烟肼、对氨基水杨酸、乙硫异烟胺和乙胺丁醇是一种可靠的测定方法。作为一种药敏试验，它得出的结果以敏感或耐药程度进行定量表达，因此仅推荐在没有配备更全面检测设备的实验室中使用。该方法还可用于确定患者血清对其自身结核分枝杆菌的抑菌活性水平。
