Fang F S, Burris R H
J Bacteriol. 1968 Aug;96(2):298-305. doi: 10.1128/jb.96.2.298-305.1968.
A c-type cytochrome from Hydrogenomonas eutropha was purified 150-fold by butanol extraction, ammonium sulfate precipitation, and column chromatography. Three distinct c-type cytochromes were recovered which did not bind with either carbon monoxide or cyanide and hence did not appear to be denatured. Polyacrylamide gel electrophoresis indicated that one protein was acidic and the other two were basic. The acidic cytochrome c had a sedimentation coefficient of 3.46. Its amino acid composition was not markedly different from other bacterial cytochromes, but relative to mammalian cytochromes c it was low in lysine, threonine, and isoleucine and high in alanine and valine.
通过丁醇萃取、硫酸铵沉淀和柱色谱法，将真养产碱菌的一种c型细胞色素纯化了150倍。回收了三种不同的c型细胞色素，它们既不与一氧化碳也不与氰化物结合，因此似乎没有变性。聚丙烯酰胺凝胶电泳表明，一种蛋白质呈酸性，另外两种呈碱性。酸性细胞色素c的沉降系数为3.46。其氨基酸组成与其他细菌细胞色素没有明显差异，但相对于哺乳动物细胞色素c，它的赖氨酸、苏氨酸和异亮氨酸含量较低，丙氨酸和缬氨酸含量较高。
