Kamihara T
J Bacteriol. 1969 Jan;97(1):151-5. doi: 10.1128/jb.97.1.151-155.1969.
The growth of Streptococcus faecalis is inhibited by propionate, and the inhibition is reversed by lipoic acid or acetate. A study of the role of pyruvate oxidation in S. faecalis showed that propionate inhibited the lipoic acid-dependent aerobic oxidation of pyruvate in resting cells. Pyruvate dehydrogenation with neotetrazolium as a hydrogen acceptor in cell-free extracts also required lipoic acid and was markedly inhibited by propionyl phosphate as well as sodium propionate. Some lipid substances, such as palmitate, oleate, behenate, and lecithin, had a lipoic acid-replacing effect on growth of the organism. Biotin or bicarbonate promoted the lipoic acid-dependent growth. Acetate-2-(14)C added to the medium was mainly incorporated into the lipid fraction of the cells. Evolution of (14)CO(2) from pyruvate-2-(14)C was not observed in resting cells of the organism, even under aerobic conditions. From the above findings, it is concluded that lipid synthesis through pyruvate oxidation plays a very important role in bacterial growth in medium containing propionate.
粪链球菌的生长受到丙酸盐的抑制，而这种抑制作用可被硫辛酸或乙酸盐逆转。一项关于丙酮酸氧化在粪链球菌中作用的研究表明，丙酸盐抑制静息细胞中依赖硫辛酸的丙酮酸需氧氧化。在无细胞提取物中以新四氮唑作为氢受体的丙酮酸脱氢反应也需要硫辛酸，并且明显受到丙酰磷酸以及丙酸钠的抑制。一些脂质物质，如棕榈酸盐、油酸盐、山嵛酸盐和卵磷脂，对该生物体的生长具有硫辛酸替代作用。生物素或碳酸氢盐促进依赖硫辛酸的生长。添加到培养基中的乙酸 - 2 -（14）C主要掺入细胞的脂质部分。即使在有氧条件下，在该生物体的静息细胞中也未观察到丙酮酸 - 2 -（14）C释放出（14）CO₂。根据上述发现，得出结论：通过丙酮酸氧化进行的脂质合成在含有丙酸盐的培养基中细菌生长中起着非常重要的作用。
