糖皮质激素对脂肪细胞膜空壳中花生四烯酸分布及动员的影响
The effects of glucocorticoids on the distribution and mobilisation of arachidonic acid in fat cell ghosts.
作者信息
Lewis G P, Piper P J, Vigo C
出版信息
Br J Pharmacol. 1979 Nov;67(3):393-400.
1 The prostaglandin synthesizing enzymes were found to be present in fat cell ghosts isolated from rabbit adipose tissue. 2 Prostaglandin E2 (PGE2) and PGF2, were synthesized by ghosts after stimulation with adrenocorticotrophic hormone (ACTH). 3 Indomethacin was found to inhibit this synthesis but not the synthesis of lipoxygenase products. 4 When fat cell ghosts were stimulated by ACTH, fatty acid release was observed from both neutral lipids and phospholipids. 5 The arachidonic acid (AA) pool within the ghosts and identified: approximately 90% was present in the phospholipid fraction, 8.5% in the neutral lipids and 1.5% unbound. 6 The glucocorticoids were found to stimulate incorporation of [14C]-AA into neutral lipids and inhibit its incorporation into phospholipids. 7 When fatty acid release was stimulated with ACTH, the glucocorticoids were found to inhibit the mobilisation of [14C]-AA from the phospholipids and enhance its release from the neutral lipids. 8 The glucocorticoids inhibit prostaglandin formation in fat cell ghosts.
发现前列腺素合成酶存在于从兔脂肪组织分离出的脂肪细胞空壳中。
促肾上腺皮质激素（ACTH）刺激后，脂肪细胞空壳合成了前列腺素E2（PGE2）和前列腺素F2α。
发现吲哚美辛可抑制这种合成，但不抑制脂氧合酶产物的合成。
当脂肪细胞空壳受到ACTH刺激时，观察到中性脂质和磷脂均有脂肪酸释放。
确定了脂肪细胞空壳内的花生四烯酸（AA）池：约90%存在于磷脂部分，8.5%存在于中性脂质中，1.5%为游离状态。
发现糖皮质激素可刺激[14C]-AA掺入中性脂质，并抑制其掺入磷脂。
当用ACTH刺激脂肪酸释放时，发现糖皮质激素可抑制[14C]-AA从磷脂中的动员，并增强其从中性脂质中的释放。
糖皮质激素抑制脂肪细胞空壳中前列腺素的形成。
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