在低离子强度下通过沉降法从单链核糖核酸中分离复制中间体。
Separation of replicative intermediate from single-stranded ribonucleic acid by sedimentation at low ionic strength.
作者信息
Fenwick M L
出版信息
J Virol. 1969 Apr;3(4):445-7. doi: 10.1128/JVI.3.4.445-447.1969.
Abstract
By sedimentation in 2.5 x 10(-4)m sodium ethylenediaminetetraacetate at 25 C, replicative intermediate could be separated from single-stranded ribonucleic acid, both viral and ribosomal. All of the label in the replicative intermediate after a brief pulse of titrated uridine was resistant to ribonuclease.
摘要
在25℃下于2.5×10⁻⁴m乙二胺四乙酸钠中进行沉降，可将复制中间体与病毒和核糖体的单链核糖核酸分离。在短暂脉冲加入滴定的尿苷后，复制中间体中的所有标记物都对核糖核酸酶具有抗性。
相似文献
1
Separation of replicative intermediate from single-stranded ribonucleic acid by sedimentation at low ionic strength.在低离子强度下通过沉降法从单链核糖核酸中分离复制中间体。
J Virol. 1969 Apr;3(4):445-7. doi: 10.1128/JVI.3.4.445-447.1969.
2
Denaturation and renaturation of viral ribonucleic acd. II. Characterization of the products resulting from annealing R17 ribonucleic acid with denatured replicative form or with denatured replicative intermediate.病毒核糖核酸的变性与复性。II. 用变性复制型或变性复制中间体使R17核糖核酸退火所产生产物的特性
J Virol. 1967 Aug;1(4):804-9. doi: 10.1128/JVI.1.4.804-809.1967.
3
Denaturation and renaturation of viral ribonucleic acid. I. Annealing R17 ribonucleic acid with denatured replicative form or with denatured replicative intermediate.病毒核糖核酸的变性与复性。I. 用变性复制型或变性复制中间体使R17核糖核酸退火
J Virol. 1967 Aug;1(4):793-803. doi: 10.1128/JVI.1.4.793-803.1967.
4
5
Fractionation of viral-specific ribonucleic acid by cesium sulfate equilibrium density-gradient centrifugation.通过硫酸铯平衡密度梯度离心法对病毒特异性核糖核酸进行分级分离。
J Mol Biol. 1966 Jul;18(2):372-81. doi: 10.1016/s0022-2836(66)80254-1.
6
Characterization of influenza virus ribonucleic acid duplex produced by annealing in vitro.体外退火产生的流感病毒核糖核酸双链体的特性分析。
J Virol. 1969 Mar;3(3):318-25. doi: 10.1128/JVI.3.3.318-325.1969.
7
Control of phage and host ribonucleic acid synthesis in phage T4 infected Escherichia coli.噬菌体T4感染的大肠杆菌中噬菌体和宿主核糖核酸合成的控制
Virology. 1968 Oct;36(2):193-200. doi: 10.1016/0042-6822(68)90136-0.
8
Replication of bacteriophage ribonucleic acid: alterations in polyribosome patterns and association of double-stranded RNA with polyribosomes in Escherichia coli infected with bacteriophage R17.噬菌体核糖核酸的复制：感染噬菌体R17的大肠杆菌中多核糖体模式的改变以及双链RNA与多核糖体的关联
Proc Natl Acad Sci U S A. 1967 Aug;58(2):743-9. doi: 10.1073/pnas.58.2.743.
9
Association of 5S ribonucleic acid to 50S ribosomal subunits of Escherichia coli and Bacillus subtilis.5S核糖核酸与大肠杆菌和枯草芽孢杆菌50S核糖体亚基的结合
Biochemistry. 1968 Mar;7(3):1141-52. doi: 10.1021/bi00843a035.
10
Studies on the biosynthesis of TMV. 3. Isolation and characterization of the replicative form and the replicative intermediate RNA.烟草花叶病毒生物合成的研究。3. 复制型和复制中间RNA的分离与特性分析
Mol Gen Genet. 1970;109(3):246-56. doi: 10.1007/BF00267013.
引用本文的文献
1
Ribonucleic acid synthesis in rinderpest virus infected cells.
Med Microbiol Immunol. 1974;160(2-3):133-41. doi: 10.1007/BF02121720.
本文引用的文献
1
The effect of reaction with formaldehyde on the sedimentation rates of ribonucleic acids.甲醛反应对核糖核酸沉降速率的影响。
Biochem J. 1968 May;107(6):851-9. doi: 10.1042/bj1070851.
2
REPLICATION OF BACTERIOPHAGE RNA: STUDIES ON THE FATE OF PARENTAL RNA.噬菌体RNA的复制：亲代RNA命运的研究
J Mol Biol. 1964 Dec;10:519-29. doi: 10.1016/s0022-2836(64)80070-x.
3
REPLICATION OF THE RNA OF BACTERIOPHAGE R17.噬菌体R17的RNA复制
Science. 1964 Oct 23;146(3643):527-30. doi: 10.1126/science.146.3643.527.
4
Replication of bacteriophage ribonucleic acid: some properties of native and denatured replicative intermediate.噬菌体核糖核酸的复制：天然和变性复制中间体的一些特性
J Virol. 1967 Jun;1(3):514-22. doi: 10.1128/JVI.1.3.514-522.1967.
5
Replication of bacteriophage ribonucleic acid: some physical properties of single-stranded, double-stranded, and branched viral ribonucleic acid.噬菌体核糖核酸的复制：单链、双链和分支病毒核糖核酸的一些物理性质
J Virol. 1967 Feb;1(1):64-75. doi: 10.1128/JVI.1.1.64-75.1967.
6
Replication of RNA viruses.RNA病毒的复制
Annu Rev Microbiol. 1968;22:305-22. doi: 10.1146/annurev.mi.22.100168.001513.
7
Purification and properties of poliovirus double-stranded ribonucleic acid.脊髓灰质炎病毒双链核糖核酸的纯化及性质
J Mol Biol. 1966 Jul;18(3):421-8. doi: 10.1016/s0022-2836(66)80034-7.
Zotero 插件