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4
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Replication of bacteriophage ribonucleic acid: some physical properties of single-stranded, double-stranded, and branched viral ribonucleic acid.噬菌体核糖核酸的复制：单链、双链和分支病毒核糖核酸的一些物理性质
J Virol. 1967 Feb;1(1):64-75. doi: 10.1128/JVI.1.1.64-75.1967.
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Purification and properties of poliovirus double-stranded ribonucleic acid.脊髓灰质炎病毒双链核糖核酸的纯化及性质
J Mol Biol. 1966 Jul;18(3):421-8. doi: 10.1016/s0022-2836(66)80034-7.

在低离子强度下通过沉降法从单链核糖核酸中分离复制中间体。

Separation of replicative intermediate from single-stranded ribonucleic acid by sedimentation at low ionic strength.

作者信息

Fenwick M L

出版信息

J Virol. 1969 Apr;3(4):445-7. doi: 10.1128/JVI.3.4.445-447.1969.

DOI:10.1128/JVI.3.4.445-447.1969
PMID:4977167
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC375790/
Abstract

By sedimentation in 2.5 x 10(-4)m sodium ethylenediaminetetraacetate at 25 C, replicative intermediate could be separated from single-stranded ribonucleic acid, both viral and ribosomal. All of the label in the replicative intermediate after a brief pulse of titrated uridine was resistant to ribonuclease.

摘要

在25℃下于2.5×10⁻⁴m乙二胺四乙酸钠中进行沉降，可将复制中间体与病毒和核糖体的单链核糖核酸分离。在短暂脉冲加入滴定的尿苷后，复制中间体中的所有标记物都对核糖核酸酶具有抗性。