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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Cleland M E, Bannister D W

Br Poult Sci. 1979 May;20(3):307-10. doi: 10.1080/00071667908416584.

The reproducibility of an enzymic method for assaying chick liver glycogen was found to be +/- 1.6%. 2. Storage of liver at either -21 or -70 degrees C for up to 4 weeks, after freezing in liquid nitrogen, had no significant effect on glycogen concentration. However, the concentration of free glucose increased by about 50% during this time. 3. Storage of liver in ice-cold saline for up to 3 h proved unsatisfactory because of a significant loss of glycogen. 4. There was no significant difference in immediate glycogen concentration in liver tissue that was freeze-clamped, frozen in liquid nitrogen or collected into ice-cold saline. The free glucose concentration was higher, when estimated immediately after, with the latter two methods of collection of liver tissue.

测定鸡肝糖原的酶法重现性为±1.6%。2. 在液氮中冷冻后，肝脏在-21℃或-70℃下储存长达4周，对糖原浓度无显著影响。然而，在此期间游离葡萄糖浓度增加了约50%。3. 将肝脏保存在冰冷盐水中长达3小时，结果并不理想，因为糖原大量损失。4. 经冷冻钳夹、在液氮中冷冻或收集到冰冷盐水中的肝组织，其即时糖原浓度无显著差异。采用后两种肝组织收集方法时，即时测定的游离葡萄糖浓度较高。

Cleland M E, Bannister D W

Br Poult Sci. 1979 May;20(3):307-10. doi: 10.1080/00071667908416584.

The reproducibility of an enzymic method for assaying chick liver glycogen was found to be +/- 1.6%. 2. Storage of liver at either -21 or -70 degrees C for up to 4 weeks, after freezing in liquid nitrogen, had no significant effect on glycogen concentration. However, the concentration of free glucose increased by about 50% during this time. 3. Storage of liver in ice-cold saline for up to 3 h proved unsatisfactory because of a significant loss of glycogen. 4. There was no significant difference in immediate glycogen concentration in liver tissue that was freeze-clamped, frozen in liquid nitrogen or collected into ice-cold saline. The free glucose concentration was higher, when estimated immediately after, with the latter two methods of collection of liver tissue.

测定鸡肝糖原的酶法重现性为±1.6%。2. 在液氮中冷冻后，肝脏在-21℃或-70℃下储存长达4周，对糖原浓度无显著影响。然而，在此期间游离葡萄糖浓度增加了约50%。3. 将肝脏保存在冰冷盐水中长达3小时，结果并不理想，因为糖原大量损失。4. 经冷冻钳夹、在液氮中冷冻或收集到冰冷盐水中的肝组织，其即时糖原浓度无显著差异。采用后两种肝组织收集方法时，即时测定的游离葡萄糖浓度较高。