Giacomoni D
Cancer Res. 1979 Nov;39(11):4481-4.
Hybrids of BALB/c lymphocytes and a murine myeloma, a tumor that expresses intracisternal A-particles, were obtained with polyethylene glycol as the fusogen. The karyotype, tumorigenicity, and A-particle expression of the hybrid clones were assessed. All the hybrid clones analyzed were tumorigenic and expressed intracisternal A-particles even when they were the result of a fusion event between two lymphocytes and one myeloma cell in which no loss of chromosomes was detected. The tumors that developed following inoculation of hybrid cells into BALB/c mice (1 x 10(6) cells/mouse) were karyotypically identical to the inoculated cells. It appears that the two myeloma cell phenotypic traits analyzed (tumorigenicity and A-particle expression) are dominant.
以聚乙二醇作为细胞融合剂，获得了BALB/c淋巴细胞与一种表达核内A颗粒的小鼠骨髓瘤的杂交细胞。对杂交克隆的核型、致瘤性和A颗粒表达进行了评估。所有分析的杂交克隆都具有致瘤性并表达核内A颗粒，即使它们是两个淋巴细胞与一个骨髓瘤细胞融合的结果，且未检测到染色体丢失。将杂交细胞接种到BALB/c小鼠（1×10⁶个细胞/只小鼠）体内后形成的肿瘤，其核型与接种的细胞相同。看来所分析的骨髓瘤细胞的两个表型特征（致瘤性和A颗粒表达）是显性的。
