Kapp D S, Hahn G M
Cancer Res. 1979 Nov;39(11):4630-5.
The effect of various sulfhydryl compounds on the survival of exponentially growing monolayer cultures of Chinese hamster cells (HA1) heated to temperatures of 37-43 degrees was examined. Concentrations of cysteamine which were nontoxic or minimally toxic at room temperature or 37 degrees became increasingly toxic at elevated temperatures, greatly potentiating the killing produced by heat alone in the absence of cysteamine. This enhancement of hyperthermia-induced cell killing increased with increasing cysteamine concentration, increasing duration of cysteamine exposure, and increasing temperature. Studies with synchronized Chinese hamster cells heated at 43 degrees for 1 hr in the presence of 16 mM cysteamine demonstrated that the potentiation of heat killing occurred in all phases of the cell cycle. Similarly, enhancement of hyperthermia-induced cell killing was seen for asynchronous cells exposed to 2-amino-ethylisothiourium bromides and cysteine, but the magnitude of the effect differed for the various sulfhydryl compounds.
研究了各种巯基化合物对加热至37 - 43摄氏度的指数生长的中国仓鼠细胞（HA1）单层培养物存活的影响。在室温或37摄氏度时无毒或毒性极小的半胱胺浓度，在升高温度时毒性越来越大，极大地增强了在无半胱胺情况下仅由热产生的杀伤作用。热疗诱导的细胞杀伤增强随着半胱胺浓度的增加、半胱胺暴露时间的延长以及温度的升高而增加。对在16 mM半胱胺存在下于43摄氏度加热1小时的同步化中国仓鼠细胞的研究表明，热杀伤的增强发生在细胞周期的所有阶段。同样，对于暴露于2 - 氨基 - 乙基异硫脲溴化物和半胱氨酸的异步细胞，也观察到热疗诱导的细胞杀伤增强，但不同巯基化合物的作用程度有所不同。
