Yagil E, Silberstein N
J Bacteriol. 1969 Dec;100(3):1364-70. doi: 10.1128/jb.100.3.1364-1370.1969.
The decay of alkaline phosphatase messenger ribonucleic acid (mRNA) in Escherichia coli was studied in cells sensitized to actinomycin D by ethylenediaminetetra-acetate treatment. It was found that in the wild-type strain (K-10) as well as in a nonsense mutant (S26C200), which is phenotypically reversible by treatment with 5-fluorouracil, mRNA decays with a half-life of 2.0 to 2.5 min. Similarly, in a 3-min pulse of labeled 5-fluorouracil, 70% of the incorporated analogue is contained in the labile RNA fraction decomposing with a half-life of 1.8 min.
在经乙二胺四乙酸处理而对放线菌素D敏感的大肠杆菌细胞中，研究了碱性磷酸酶信使核糖核酸（mRNA）的衰变情况。结果发现，在野生型菌株（K - 10）以及一个无义突变体（S26C200）中，该突变体在用5 - 氟尿嘧啶处理后表型可逆转，mRNA的半衰期为2.0至2.5分钟。同样，在3分钟的标记5 - 氟尿嘧啶脉冲实验中，70%掺入的类似物存在于半衰期为1.8分钟的不稳定RNA组分中。
