大肠杆菌中碱性磷酸酶的正常信使核糖核酸和5-氟尿嘧啶取代信使核糖核酸的衰变
Decay of normal and 5-fluorouracil-substituted messenger ribonucleic acid of alkaline phosphatase in Escherichia coli.
作者信息
Yagil E, Silberstein N
出版信息
J Bacteriol. 1969 Dec;100(3):1364-70. doi: 10.1128/jb.100.3.1364-1370.1969.
Abstract
The decay of alkaline phosphatase messenger ribonucleic acid (mRNA) in Escherichia coli was studied in cells sensitized to actinomycin D by ethylenediaminetetra-acetate treatment. It was found that in the wild-type strain (K-10) as well as in a nonsense mutant (S26C200), which is phenotypically reversible by treatment with 5-fluorouracil, mRNA decays with a half-life of 2.0 to 2.5 min. Similarly, in a 3-min pulse of labeled 5-fluorouracil, 70% of the incorporated analogue is contained in the labile RNA fraction decomposing with a half-life of 1.8 min.
摘要
在经乙二胺四乙酸处理而对放线菌素D敏感的大肠杆菌细胞中，研究了碱性磷酸酶信使核糖核酸（mRNA）的衰变情况。结果发现，在野生型菌株（K - 10）以及一个无义突变体（S26C200）中，该突变体在用5 - 氟尿嘧啶处理后表型可逆转，mRNA的半衰期为2.0至2.5分钟。同样，在3分钟的标记5 - 氟尿嘧啶脉冲实验中，70%掺入的类似物存在于半衰期为1.8分钟的不稳定RNA组分中。
相似文献
1
Decay of normal and 5-fluorouracil-substituted messenger ribonucleic acid of alkaline phosphatase in Escherichia coli.大肠杆菌中碱性磷酸酶的正常信使核糖核酸和5-氟尿嘧啶取代信使核糖核酸的衰变
J Bacteriol. 1969 Dec;100(3):1364-70. doi: 10.1128/jb.100.3.1364-1370.1969.
2
Effect of actinomycin on the synthesis of macromolecules in Escherichia coli.放线菌素对大肠杆菌中大分子合成的影响。
Biochim Biophys Acta. 1966 Apr 18;119(1):200-3. doi: 10.1016/0005-2787(66)90053-0.
3
Messenger RNA turnover and protein synthesis in actinomycin inhibited Escherichia coli.放线菌素抑制的大肠杆菌中的信使核糖核酸周转和蛋白质合成。
Biochem Biophys Res Commun. 1965 Dec 9;21(5):404-12. doi: 10.1016/0006-291x(65)90396-7.
4
[Destruction of total messenger RNA in E. coli by treatment with actinomycin D and rifampicin].
Vopr Med Khim. 1974 Jul-Aug;20(4):388-92.
5
6
Regulation of ribonucleic acid synthesis in Escherichia coli during diauxie lag: accumulation of heterogeneous ribonucleic acid.大肠杆菌在二次生长延滞期核糖核酸合成的调节：不均一核糖核酸的积累
J Bacteriol. 1970 Jun;102(3):740-6. doi: 10.1128/jb.102.3.740-746.1970.
7
Decay of messenger ribonucleic acid from the lactose operon of Escherichia coli as a function of growth temperature.大肠杆菌乳糖操纵子中信使核糖核酸的衰变与生长温度的关系。
J Mol Biol. 1973 Feb 15;74(1):21-31. doi: 10.1016/0022-2836(73)90351-3.
8
Messenger ribonucleic acid stability in relaxed and stringent Escherichia coli starved for methionine.在缺乏蛋氨酸的松弛型和严谨型大肠杆菌中信使核糖核酸的稳定性
J Bacteriol. 1973 Jul;115(1):447-9. doi: 10.1128/jb.115.1.447-449.1973.
9
Amino acid regulation of messenger ribonucleic acid synthesis in T4-infected Escherichia coli.T4 噬菌体感染的大肠杆菌中信使核糖核酸合成的氨基酸调控
J Bacteriol. 1969 Jan;97(1):313-21. doi: 10.1128/jb.97.1.313-321.1969.
10
Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli.大肠杆菌中β-半乳糖苷酶信使核糖核酸的稳定性
J Bacteriol. 1965 Dec;90(6):1611-6. doi: 10.1128/jb.90.6.1611-1616.1965.
引用本文的文献
1
Histone-induced changes of protein synthesis in Escherichia coli. II. Effect of histone on alkaline phosphatase synthesis.组蛋白诱导的大肠杆菌蛋白质合成变化。II. 组蛋白对碱性磷酸酶合成的影响。
Folia Microbiol (Praha). 1973;18(5):368-75. doi: 10.1007/BF02875931.
2
Pleiotropic effects of mutations involved in the regulation of Escherichia coli K-12 alkaline phosphatase.参与大肠杆菌K-12碱性磷酸酶调控的突变的多效性效应。
J Bacteriol. 1974 Aug;119(2):583-92. doi: 10.1128/jb.119.2.583-592.1974.
本文引用的文献
1
Massive incorporation of 5-fluorouracil into a bacterial ribonucleic acid.5-氟尿嘧啶大量掺入细菌核糖核酸中。
Nature. 1959 Oct 17;184:1213-5. doi: 10.1038/1841213a0.
2
CHARACTERISTICS OF 5-FLUOROURACIL-INDUCED SYNTHESIS OF ALKALINE PHOSPHATASE.5-氟尿嘧啶诱导碱性磷酸酶合成的特征
J Mol Biol. 1965 Apr;11:845-50. doi: 10.1016/s0022-2836(65)80042-0.
3
A NONSPECIFIC INCREASE IN PERMEABILITY IN ESCHERICHIA COLI PRODUCED BY EDTA.乙二胺四乙酸（EDTA）导致大肠杆菌通透性非特异性增加。
Proc Natl Acad Sci U S A. 1965 Apr;53(4):745-50. doi: 10.1073/pnas.53.4.745.
4
THE ROLES OF INDUCER AND CATABOLITE REPRESSOR IN THE SYNTHESIS OF BETA-GALACTOSIDASE BY ESCHERICHIA COLI.诱导物和分解代谢物阻遏物在大肠杆菌β-半乳糖苷酶合成中的作用
J Mol Biol. 1964 Jan;8:105-27. doi: 10.1016/s0022-2836(64)80153-4.
5
KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA.诱导酶合成动力学。半乳糖苷酶特异性信使核糖核酸平均寿命的测定。
Biochim Biophys Acta. 1963 Oct 15;76:293-309.
6
THE MOLECULAR BASIS OF HISTIDASE INDUCTION IN BACILLUS SUBTILIS.枯草芽孢杆菌中组氨酸酶诱导的分子基础
J Mol Biol. 1963 Oct;7:401-20. doi: 10.1016/s0022-2836(63)80033-9.
7
Fingerprint analysis of alkaline phosphatase of Escherichia coli K12.大肠杆菌K12碱性磷酸酶的指纹分析
J Mol Biol. 1963 Apr;6:330-40. doi: 10.1016/s0022-2836(63)80092-3.
8
The composition of tobacco mosaic virus protein after the incorporation of 5-fluorouracil into the virus.
J Mol Biol. 1963 Feb;6:164-6. doi: 10.1016/s0022-2836(63)80133-3.
9
Suppression of mutations in the alkaline phosphatase structural cistron of E. coli.大肠杆菌碱性磷酸酶结构顺反子中突变的抑制
Proc Natl Acad Sci U S A. 1962 Jul 15;48(7):1121-7. doi: 10.1073/pnas.48.7.1121.
10
Genetic control of induction of alkaline phosphatase synthesis in E. coli.大肠杆菌中碱性磷酸酶合成诱导的遗传控制。
Proc Natl Acad Sci U S A. 1962 Aug;48(8):1398-402. doi: 10.1073/pnas.48.8.1398.
Zotero 插件