标准化病毒血凝和血凝抑制试验。I. 红细胞悬液的标准化。
Standardized viral hemagglutination and hemagglutination-inhibition tests. I. Standardization of erythrocyte suspensions.
作者信息
Hierholzer J C, Suggs M T
出版信息
Appl Microbiol. 1969 Nov;18(5):816-23. doi: 10.1128/am.18.5.816-823.1969.
Abstract
A spectrophotometric procedure for standardizing red blood cell suspensions for use in viral hemagglutination tests is described. The procedure is based on highly reproducible cyanmethemoglobin absorbance readings at 540 nm on any suitable spectrophotometer. Target values for milligrams of cyanmethemoglobin per 100 ml are given for the red cell suspensions of all mammalian and avian species employed in viral hemagglutination tests. By use of these values and a cyanmethemoglobin standard curve for a particular photometer, approximate 4% erythrocyte suspensions can be diluted to any lesser concentration. Coefficients of variation for the various diluted suspensions range from 4 to 7%.
摘要
本文描述了一种用于病毒血凝试验标准化红细胞悬液的分光光度法。该方法基于在任何合适的分光光度计上，于540nm处对氰化高铁血红蛋白进行高度可重复的吸光度读数。文中给出了用于病毒血凝试验的所有哺乳动物和鸟类红细胞悬液每100ml中氰化高铁血红蛋白毫克数的目标值。利用这些值以及特定光度计的氰化高铁血红蛋白标准曲线，可将约4%的红细胞悬液稀释至任何较低浓度。各种稀释悬液的变异系数范围为4%至7%。
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