Mettler N E, Clarke D H, Casals J
Appl Microbiol. 1971 Sep;22(3):377-9. doi: 10.1128/am.22.3.377-379.1971.
Antigens for Grand Arbaud, Hazara, and California arboviruses were able to agglutinate goose and either dog, hamster and guinea pig, or hamster red blood cells (RBC) to the same titer at the same pH; in hemagglutination-inhibition (HI) tests, titers for homologous and related sera were the same with these different types of RBC or occasionally one dilution higher with the mammalian cells. Antigens for St. Louis encephalitis and Eastern equine encephalitis viruses required use of lower antigen dilutions with human, guinea pig, and hamster RBC than with goose RBC. The results of comparative HI testing with these latter antigens and types of RBC indicate that HI titer is not directly related to the antigen dilution used with different types of RBC.
格兰德阿尔博德病毒、哈扎拉病毒和加利福尼亚虫媒病毒的抗原,在相同pH值下,能够使鹅红细胞以及狗、仓鼠和豚鼠的红细胞,或仓鼠红细胞凝集至相同滴度;在血凝抑制(HI)试验中,对于同源和相关血清,使用这些不同类型红细胞时的滴度相同,或偶尔在使用哺乳动物细胞时高一个稀释度。圣路易斯脑炎病毒和东部马脑炎病毒的抗原,与人、豚鼠和仓鼠红细胞相比,与鹅红细胞一起使用时需要更低的抗原稀释度。使用后一种抗原和红细胞类型进行比较HI试验的结果表明,HI滴度与不同类型红细胞所使用的抗原稀释度没有直接关系。