莱茵衣藻突变株ac - 20中的叶绿体结构与功能。I. 二氧化碳固定及1,5 - 二磷酸核酮糖羧化酶合成
Chloroplast structure and function in ac-20, a mutant strain of Chlamydomonas reinhardi. I. CO2 fixation and ribulose-1,5-diphosphate carboxylase synthesis.
作者信息
Togasaki R K, Levine R P
出版信息
J Cell Biol. 1970 Mar;44(3):531-9. doi: 10.1083/jcb.44.3.531.
Abstract
A mutant strain of the green alga Chlamydomonas reinhardi, ac-20, is described in which both the rate of CO(2) fixation by whole cells and the rate of carboxylation of ribulose-1,5-diphosphate in cell-free extracts are reduced, particularly when sodium acetate is present in the growth medium. Of the enzymes of the reductive pentose phosphate cycle tested, only ribulose-1,5-diphosphate carboxylase activity is reduced in the mutant strain, and it appears that the low carboxylase activity limits the strain's rate of photosynthetic carbon metabolism. Evidence is presented to show that the fluctuation in the level of the enzyme activity in the presence or absence of acetate results from the fluctuation in the level of some factor(s) limiting the rate of synthesis of the protein.
摘要
描述了莱茵衣藻的一种突变株ac - 20，其中全细胞固定CO₂的速率以及无细胞提取物中1,5 - 二磷酸核酮糖的羧化速率均降低，尤其是当生长培养基中存在乙酸钠时。在所测试的还原性戊糖磷酸循环的酶中，只有突变株中的1,5 - 二磷酸核酮糖羧化酶活性降低，并且似乎低羧化酶活性限制了该菌株的光合碳代谢速率。有证据表明，在有或没有乙酸盐存在的情况下，酶活性水平的波动是由限制蛋白质合成速率的某些因素水平的波动引起的。
相似文献
1
Chloroplast structure and function in ac-20, a mutant strain of Chlamydomonas reinhardi. I. CO2 fixation and ribulose-1,5-diphosphate carboxylase synthesis.莱茵衣藻突变株ac - 20中的叶绿体结构与功能。I. 二氧化碳固定及1,5 - 二磷酸核酮糖羧化酶合成
J Cell Biol. 1970 Mar;44(3):531-9. doi: 10.1083/jcb.44.3.531.
2
Chloroplast structure and function in ac-20, a mutant strain of Chlamydomonas reinhardi. 3. Chloroplast ribosomes and membrane organization.莱茵衣藻突变株ac - 20中的叶绿体结构与功能。3. 叶绿体核糖体与膜组织
J Cell Biol. 1970 Mar;44(3):547-62. doi: 10.1083/jcb.44.3.547.
3
Chloroplast structure and function in ac-20, a mutant strain of Chlamydomonas reinhardi. II. Photosynthetic electron transport.莱茵衣藻突变株ac-20中的叶绿体结构与功能。II. 光合电子传递。
J Cell Biol. 1970 Mar;44(3):540-6. doi: 10.1083/jcb.44.3.540.
4
A mutant strain of Chlamydomonas reinhardi exhibiting altered ribulosebisphosphate carboxylase.莱茵衣藻的一种突变菌株，其核酮糖二磷酸羧化酶表现出改变。
Eur J Biochem. 1976 Jan 15;61(2):465-74. doi: 10.1111/j.1432-1033.1976.tb10040.x.
5
Ribulose 1,5-diphosphate carboxylase and Cholorobium thiosulfatophilum.核酮糖1,5 - 二磷酸羧化酶与嗜硫代硫酸盐绿菌
Arch Microbiol. 1976 Aug;109(1-2):15-9. doi: 10.1007/BF00425107.
6
Chloroplast ribosome deficient mutants in the green alga Chlamydomonas reinhardi and the question of chloroplast ribosome function.莱茵衣藻中叶绿体核糖体缺陷型突变体及叶绿体核糖体功能问题
J Cell Sci. 1972 Mar;10(2):267-305. doi: 10.1242/jcs.10.2.267.
7
Characterization of the oxygenase activity in a mutant of Chlamydomonas reinhardi exhibiting altered ribulosebisphosphate carboxylase.莱茵衣藻一个表现出核酮糖二磷酸羧化酶改变的突变体中加氧酶活性的表征
Eur J Biochem. 1976 Jan 15;61(2):475-80. doi: 10.1111/j.1432-1033.1976.tb10041.x.
8
A mutant strain of Chlamydomonas reinhardi lacking ribulose diphosphate carboxylase activity.莱茵衣藻的一种缺乏核酮糖二磷酸羧化酶活性的突变菌株。
Proc Natl Acad Sci U S A. 1965 May;53(5):987-90. doi: 10.1073/pnas.53.5.987.
9
A mutant strain of Scenedesmus obliquus deficient in ribulose diphosphate carboxylase, cytochrome f and photosystem II activity.一种斜生栅藻突变株，缺乏二磷酸核酮糖羧化酶、细胞色素f和光系统II活性。
Biochim Biophys Acta. 1976 Jan 15;423(1):65-79. doi: 10.1016/0005-2728(76)90101-8.
10
Cell-free synthesis of active ribulose-1,5-bisphosphate carboxylase in the mesophyll chloroplasts of Sorghum vulgare.在高粱叶肉叶绿体中无细胞合成活性核酮糖-1,5-二磷酸羧化酶
Biochim Biophys Acta. 1980;606(1):83-94. doi: 10.1016/0005-2787(80)90100-8.
引用本文的文献
1
Remembering Robert (Bob) Togasaki (1932-2019): A leader in Chlamydomonas genetics and in plant biology, as well as a teacher par excellence.纪念罗伯特·托加萨基（Robert（Bob）Togasaki）（1932-2019）：衣藻遗传学和植物生物学的领军人物，也是一位卓越的教师。
Photosynth Res. 2022 Apr;152(1):73-86. doi: 10.1007/s11120-021-00893-5. Epub 2022 Jan 13.
2
Rubulose diphosphate carboxylase synthesis in Chlamydomonas reinhardii: Inhibition by chloramphenicol and stimulation by cycloheximide.莱茵衣藻中核酮糖二磷酸羧化酶的合成：氯霉素的抑制作用和环己亚胺的刺激作用。
Planta. 1974 Jan;120(2):181-8. doi: 10.1007/BF00384928.
3
Multidisciplinary research in photosynthesis: A case history based on the green alga Chlamydomonas.光合作用的多学科研究：以绿藻衣藻为例的历史案例。
Photosynth Res. 1986 Jan;10(3):415-22. doi: 10.1007/BF00118307.
4
Ribulose bisphosphate carboxylase from a mutant strain of Chlamydomonas reinhardii deficient in chloroplast ribosomes : The absence of both subunits and their pattern of synthesis during enzyme recovery.叶绿体核糖体缺陷的莱茵衣藻突变株的核酮糖二磷酸羧化酶：在酶回收过程中两个亚基的缺失及其合成模式。
Planta. 1979 Jan;144(3):271-6. doi: 10.1007/BF00388769.
5
The ultrastructure of a Chlamydomonas reinhardtii mutant strain lacking phytoene synthase resembles that of a colorless alga.缺少八氢番茄红素合酶的莱茵衣藻突变株的超微结构类似于无色藻类。
Mol Plant. 2008 Nov;1(6):925-37. doi: 10.1093/mp/ssn046. Epub 2008 Sep 19.
6
Posttranscriptional Regulation of Ribulose 1,5-bisphosphate Carboxylase Small Subunit Accumulation in Chlamydomonas reinhardtii.莱茵衣藻中核酮糖 1,5-二磷酸羧化酶小亚基积累的转录后调控。
Plant Physiol. 1983 Jul;72(3):847-54. doi: 10.1104/pp.72.3.847.
7
The Site of Synthesis of Two Chloroplast Cytochromes in Chlamydomonas reinhardi.莱茵衣藻中两种叶绿体细胞色素的合成位点
Plant Physiol. 1972 Apr;49(4):661-2. doi: 10.1104/pp.49.4.661.
8
Chlorophyll, Ribulose-1,5-diphosphate Carboxylase, and Hill Reaction Activity in Developing Leaves of Populus deltoides.发育中的柳树叶片中的叶绿素、核酮糖-1,5-二磷酸羧化酶和希尔反应活性。
Plant Physiol. 1971 Aug;48(2):143-5. doi: 10.1104/pp.48.2.143.
9
In Vitro Protein Synthesis by Plastids of Phaseolus vulgaris: V. Incorporation of C-Leucine into a Protein Fraction Containing Ribulose 1,5-Diphosphate Carboxylase.菜豆质体的体外蛋白质合成：V. C-亮氨酸掺入含核酮糖 1,5-二磷酸羧化酶的蛋白质组分中。
Plant Physiol. 1970 Jul;46(1):136-41. doi: 10.1104/pp.46.1.136.
10
The effects of inhibitors of RNA and protein synthesis on chloroplast structure and function in wild-type Chlamydomonas reinhardi.RNA和蛋白质合成抑制剂对野生型莱茵衣藻叶绿体结构和功能的影响。
J Cell Biol. 1971 Jul;50(1):35-49. doi: 10.1083/jcb.50.1.35.
本文引用的文献
1
Photosynthetic Electron Transport Chain of Chlamydomonas reinhardi VI. Electron Transport in Mutant Strains Lacking Either Cytochrome 553 or Plastocyanin.莱茵衣藻的光合电子传递链VI. 缺乏细胞色素553或质体蓝素的突变株中的电子传递
Plant Physiol. 1966 Dec;41(10):1648-56. doi: 10.1104/pp.41.10.1648.
2
Regulation of Photosynthetic Capacity in Chlamydomonas mundana.mundana衣藻光合能力的调控
Plant Physiol. 1966 May;41(5):885-90. doi: 10.1104/pp.41.5.885.
3
Intracellular and Phylogenetic Distribution of Ribulose 1,5-Diphosphate Carboxylase and D-Glyceraldehyde-3-Phosphate Dehydrogenases.1,5-二磷酸核酮糖羧化酶与3-磷酸甘油醛脱氢酶的细胞内分布及系统发育分布
Plant Physiol. 1959 May;34(3):324-9. doi: 10.1104/pp.34.3.324.
4
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.分离叶绿体中的铜酶。甜菜中的多酚氧化酶。
Plant Physiol. 1949 Jan;24(1):1-15. doi: 10.1104/pp.24.1.1.
5
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
6
The influence of growth substrates on metabolic pathways in Micrococcus denitrificans.生长底物对反硝化微球菌代谢途径的影响。
Biochim Biophys Acta. 1960 Mar 25;39:9-24. doi: 10.1016/0006-3002(60)90117-7.
7
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
8
A screening technique for photosynthetic mutants in unicellular algae.一种用于单细胞藻类光合突变体的筛选技术。
Nature. 1960 Oct 22;188:339-40. doi: 10.1038/188339b0.
9
Carbon metabolism in Chromatium.嗜色菌中的碳代谢。
J Biol Chem. 1961 Jul;236:2140-9.
10
Regulatory mechanisms in carbohydrate metabolism. III. Limiting factors in glycolysis of ascites tumor cells.碳水化合物代谢中的调节机制。III. 腹水肿瘤细胞糖酵解中的限制因素。
J Biol Chem. 1959 May;234(5):1029-35.
Zotero 插件