Georges J
Clin Chem. 1979 Nov;25(11):1888-90.
I propose a single, quick method for measuring ammonia in urine and urea in plasma and urine. An ammonia-selective electrodie is used, set up on a microcell, which allows use of small sample volumes. Ammonia is measured directly after partial conversion of ammonium ions to NH3. Urea is measured after its hydrolysis by urease. With urine, the two procedures can be carried out successively in the same cell and on the same sample without changing the procedural conditions. Linear electrode response and accuracy have been checked for concentrations in the expected (normal) range.
我提出了一种单一、快速的方法来测量尿液中的氨以及血浆和尿液中的尿素。使用一种氨选择性电极，它安装在一个微电池上，这使得能够使用少量样本。在铵离子部分转化为NH3后直接测量氨。尿素在被脲酶水解后进行测量。对于尿液，这两个程序可以在同一个细胞中、对同一样本依次进行，而无需改变程序条件。已经针对预期（正常）范围内的浓度检查了电极的线性响应和准确性。
