免疫沉淀素在琼脂糖凝胶中的快速形成。

Rapid development of immunoprecipitins in agarose gel.

作者信息

Heuck C C, Erbe I, Wirth A, Schlierf G

出版信息

Clin Chim Acta. 1979 Nov 2;98(3):195-9. doi: 10.1016/0009-8981(79)90145-1.

DOI:10.1016/0009-8981(79)90145-1

Abstract

A procedure for the rapid development of immunoprecipitins in agarose gels has been developed using polyanion precipitation after immunoelectrophoretical separations. Excellent results are obtained for serum apolipoproteins, immunoglobulins and other proteins. It is shown that quantitative determination of apolipoprotein B in hyperlipemic serum and isolated lipoproteins is affected by immunoprecipitated complexes when rocket immunoelectrophoresis is used. This effect can be avoided by addition of polyvinylpyrrolidone.

摘要

已开发出一种在琼脂糖凝胶中快速形成免疫沉淀素的方法，该方法利用免疫电泳分离后的聚阴离子沉淀。血清载脂蛋白、免疫球蛋白及其他蛋白质均获得了优异的结果。结果表明，当使用火箭免疫电泳时，高脂血症血清和分离的脂蛋白中载脂蛋白B的定量测定会受到免疫沉淀复合物的影响。通过添加聚乙烯吡咯烷酮可避免这种影响。

相似文献

1

Rapid development of immunoprecipitins in agarose gel.免疫沉淀素在琼脂糖凝胶中的快速形成。

Clin Chim Acta. 1979 Nov 2;98(3):195-9. doi: 10.1016/0009-8981(79)90145-1.

2

An improved staining technique for precipitin bands in agar or agarose gels.一种用于琼脂或琼脂糖凝胶中沉淀素带的改良染色技术。

Biochem Biophys Res Commun. 1980 Oct 16;96(3):1123-7. doi: 10.1016/0006-291x(80)90068-6.

3

Quantitative determination of apolipoprotein A-I in high-density lipoproteins and 'free' apolipoprotein A-I by two-dimensional agarose gel lipoprotein-'rocket' immunoelectrophoresis of human serum.通过人血清的二维琼脂糖凝胶脂蛋白“火箭”免疫电泳法定量测定高密度脂蛋白中的载脂蛋白A-I和“游离”载脂蛋白A-I。

Biochim Biophys Acta. 1986 Nov 14;879(2):134-9. doi: 10.1016/0005-2760(86)90095-0.

4

Ultrathinfilm immunoelectrophoresis.

Ann Clin Biochem. 1982 Mar;19(Pt 2):114-6. doi: 10.1177/000456328201900210.

5

Fluorography of tritium-labelled proteins in immunoelectrophoresis.免疫电泳中氚标记蛋白质的荧光自显影法。

J Biochem Biophys Methods. 1979;1(1):59-64. doi: 10.1016/0165-022x(79)90046-0.

6

Quantitation of antigens by immunoelectrophoresis.通过免疫电泳对抗原进行定量分析。

Immunochemistry. 1974 Jun;11(6):305-8. doi: 10.1016/0019-2791(74)90366-8.

7

Electroimmunoprecipitation in agarose gels: correlation between electroendosmosis and buffer pH.琼脂糖凝胶中的电免疫沉淀：电渗与缓冲液pH值之间的相关性。

J Biochem Biophys Methods. 1982 Aug;6(3):261-5. doi: 10.1016/0165-022x(82)90048-3.

8

Discontinuous polyacrylamide gel-agarose gel immunoelectrophoresis for analysis of plasma lipoproteins.

Electrophoresis. 1990 Oct;11(10):846-51. doi: 10.1002/elps.1150111013.

9

[Example of the use of affinity electrophoresis for the determiniation of antigens].

C R Acad Hebd Seances Acad Sci D. 1976 Oct 27;283(10):1253-5.

10

Differential quantification of SIgA and SC by two-directional rocket method.采用双向火箭免疫电泳法对分泌型免疫球蛋白A（SIgA）和分泌片（SC）进行差异定量分析。

Immunology. 1980 Aug;40(4):597-604.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。