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三种细胞培养系统作为流感病毒检测底物的评估。

Evaluation of three cell culture systems as substrates for influenza virus assay.

作者信息

Goldstein M A, Tauraso N M, Orr H C

出版信息

Appl Microbiol. 1970 Apr;19(4):580-2. doi: 10.1128/am.19.4.580-582.1970.

DOI:10.1128/am.19.4.580-582.1970
PMID:4986726
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC376741/
Abstract

The propagation of various influenza virus strains in primary rhesus monkey kidney (RMK), primary hamster kidney (HK), and the MDCK-USD canine kidney (CK) cell cultures was compared. Virus-infected cultures were examined for cytopathic effect (CPE) and by performing the hemadsorption (HAd) technique. The highest HAd titers were found most often in HK, followed by RMK and CK. However, the CK cell line provided the best substrate for detecting CPE. Although influenza B strains tended to grow to higher titers, these strains produced less CPE than did the A strains.

摘要

比较了各种流感病毒株在原代恒河猴肾(RMK)、原代仓鼠肾(HK)和MDCK - USD犬肾(CK)细胞培养物中的增殖情况。对病毒感染的培养物进行细胞病变效应(CPE)检查,并采用血细胞吸附(HAd)技术进行检测。发现HK中HAd滴度最高的情况最为常见,其次是RMK和CK。然而,CK细胞系是检测CPE的最佳底物。尽管乙型流感病毒株往往能生长到更高的滴度,但这些毒株产生的CPE比甲型毒株少。

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本文引用的文献

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SERIAL MULTIPLICATION OF AN INFLUENZA VIRUS (NWS) IN CERTAIN HUMAN DIPLOID CELL STRAINS.流感病毒(NWS)在某些人二倍体细胞株中的连续增殖
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INFLUENZA VIRUSES IN CELL CULTURES. I. PREPARATION AND USE OF FETAL PIG LUNG CELLS FOR QUANTAL ASSAY.细胞培养中的流感病毒。I. 用于定量测定的胎猪肺细胞的制备与应用
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Cytocidal assay and propagation of human influenza viruses in cultured primary hamster kidney cells.人流感病毒在原代培养仓鼠肾细胞中的杀细胞试验及增殖
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Hemadsorption (adsorption-hemagglutination) test for viral agents in tissue culture with special reference to influenza.用组织培养进行病毒因子的血细胞吸附(吸附-血细胞凝集)试验,特别参考流感病毒。
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Isolation and cytopathogenic effect of influenza B viruses in monkey kidney cultures.乙型流感病毒在猴肾培养物中的分离及细胞病变效应
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