Goldstein M A, Tauraso N M, Orr H C
Appl Microbiol. 1970 Apr;19(4):580-2. doi: 10.1128/am.19.4.580-582.1970.
The propagation of various influenza virus strains in primary rhesus monkey kidney (RMK), primary hamster kidney (HK), and the MDCK-USD canine kidney (CK) cell cultures was compared. Virus-infected cultures were examined for cytopathic effect (CPE) and by performing the hemadsorption (HAd) technique. The highest HAd titers were found most often in HK, followed by RMK and CK. However, the CK cell line provided the best substrate for detecting CPE. Although influenza B strains tended to grow to higher titers, these strains produced less CPE than did the A strains.
比较了各种流感病毒株在原代恒河猴肾(RMK)、原代仓鼠肾(HK)和MDCK - USD犬肾(CK)细胞培养物中的增殖情况。对病毒感染的培养物进行细胞病变效应(CPE)检查,并采用血细胞吸附(HAd)技术进行检测。发现HK中HAd滴度最高的情况最为常见,其次是RMK和CK。然而,CK细胞系是检测CPE的最佳底物。尽管乙型流感病毒株往往能生长到更高的滴度,但这些毒株产生的CPE比甲型毒株少。