Pacold S T, Blackard W G
Endocrinology. 1979 Dec;105(6):1452-7. doi: 10.1210/endo-105-6-1452.
The presence of specific insulin receptors in homogenates of various parts of the rat brain was demonstrated. Hypothalamic preparations exhibited the greatest insulin binding. Scatchard plots of the binding data were typically curvilinear. Insulin binding to the brain homogenates was influenced by pH, incubation time, temperature, and ionic milieu. The specificity of insulin binding to the brain homogenates was demonstrated by partial displacement of labeled insulin by insulin analogs according to their biological activities and by inhibition of insulin binding by serum containing an antibody to insulin receptors. Insulin binding to brain homogenates from streptozotocin-induced diabetic rats was similar to that from control animals and failed to indicate up-regulation of these receptors.
已证实大鼠脑各部位匀浆中存在特异性胰岛素受体。下丘脑制剂表现出最大的胰岛素结合能力。结合数据的Scatchard图通常呈曲线状。胰岛素与脑匀浆的结合受pH、孵育时间、温度和离子环境的影响。根据胰岛素类似物的生物活性，通过其对标记胰岛素的部分置换，以及含有胰岛素受体抗体的血清对胰岛素结合的抑制作用，证明了胰岛素与脑匀浆结合的特异性。链脲佐菌素诱导的糖尿病大鼠脑匀浆中的胰岛素结合与对照动物相似，未能表明这些受体的上调。
