Onabanjo A O, Bhabani A R, Maegraith B G
Br J Exp Pathol. 1970 Oct;51(5):534-40.
Experiments were performed on healthy (non-infected) and -infected rhesus monkeys () to determine the activity of the kinin-destroying enzymes (kininases) present in the circulation. Methods for the studies and estimation of kininases in the circulating blood of control and -infected animals are described. The activity of the enzymes, present in the blood in all animals investigated, was evaluated from both methods employed by incubation with synthetic bradykinin. The isolated rat uterus served as the test organ. Studies were made on protein peak fractions obtained by column chromatography from sera of uninfected and infected animals. Kininases activity was present in fractions 4 and 5 of the control animals and in fractions 3, 4, 4a and 5 of the infected animals. The total mean value of kininase activity in the separated fractions of control animals was 179 enzyme units as compared with 489 units in monkeys infected with In the plasma, the pre-infection level of kininases obtained in the 3 animals studied was 0·0007-0·0008 (expressed in reciprocals of seconds); in the late stages of the infection, the level was 0·0022, with a subsequent drop of 0·0002-0·0004 at the terminal stages. It is concluded that increase in kininases activity in malarial infection in relation to the normal values obtained for circulating kinins suggested a rapid turn-over of the latter. In this case, the balance of kininases: kinins in infection thus serve as a regulatory mechanism in minimising the damage caused on the capillary endothelium.
对健康（未感染）和感染的恒河猴进行实验，以测定循环中存在的激肽破坏酶（激肽酶）的活性。描述了在对照动物和感染动物的循环血液中研究和评估激肽酶的方法。通过与合成缓激肽孵育所采用的两种方法，对所有研究动物血液中存在的酶活性进行了评估。分离的大鼠子宫用作测试器官。对通过柱色谱法从未感染和感染动物的血清中获得的蛋白质峰馏分进行了研究。对照动物的馏分4和5以及感染动物的馏分3、4、4a和5中存在激肽酶活性。对照动物分离馏分中激肽酶活性的总平均值为179酶单位，而感染的猴子为489单位。在血浆中，所研究的3只动物感染前激肽酶水平为0·0007 - 0·0008（以秒倒数表示）；在感染后期，该水平为0·0022，在终末期随后下降0·0002 - 0·0004。得出的结论是，疟疾感染中激肽酶活性相对于循环激肽的正常水平增加表明后者周转迅速。在这种情况下，感染中激肽酶与激肽的平衡因此作为一种调节机制，可将对毛细血管内皮造成的损害降至最低。
