Baechtel F S, Prager M D, Vitetta E S
Eur J Immunol. 1979 Jun;9(6):450-4. doi: 10.1002/eji.1830090607.
The specificity was established for the anti-thymocyte autoantibody which appeared in BALB/c mice (BAA) during immunization to the P 1798 lymphoma. The results indicate that BAA is specific for the Thy-1 alloantigen of murine T cells. By immunoprecipitation of 125I-labeled A/J thymocyte surface antigens from freeze-thaw lysates and electrophoretic resolution of the immunoprecipitates on sodium dodecyl sulfate polyacrylamide gels, the BAA and congenic anti-Thy-1.1 or 1.2 were shown to be specific for determinants on the same antigens. Specificity of BAA for Thy-1.2 was shown also quantitative absorption studies. Coating P 1798 lymphoma cells with either BAA or anti-Thy-1.2 reduced the absorptive capacity of these cells for the same or the reciprocal antiserum. Coating P 1798 with an antiserum to P 1798 which was indifferent to the Thy-1.2 alloantigen did not diminish the absorptive capacity of these cells for either BAA or anti-Thy-1.2. These studies have provided firm evidence that BAA is specific for determinants on the Thy-1 alloantigen.
确定了在对P 1798淋巴瘤进行免疫接种期间出现在BALB/c小鼠（BAA）体内的抗胸腺细胞自身抗体的特异性。结果表明，BAA对鼠T细胞的Thy-1同种异体抗原有特异性。通过对冻融裂解物中125I标记的A/J胸腺细胞表面抗原进行免疫沉淀，并在十二烷基硫酸钠聚丙烯酰胺凝胶上对免疫沉淀物进行电泳分离，发现BAA与同基因抗-Thy-1.1或1.2对相同抗原上的决定簇具有特异性。通过定量吸收研究也表明了BAA对Thy-1.2的特异性。用BAA或抗-Thy-1.2包被P 1798淋巴瘤细胞会降低这些细胞对相同或相互抗血清的吸收能力。用对Thy-1.2同种异体抗原无反应的抗P 1798抗血清包被P 1798不会降低这些细胞对BAA或抗-Thy-1.2的吸收能力。这些研究提供了确凿的证据，证明BAA对Thy-1同种异体抗原上的决定簇具有特异性。
